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Determinación y comparación cualitativa de cannabinoides presentes en productos naturales comerciales del departamento del Cauca-Colombia
Determination and qualitative comparison of cannabinoids present in commercial natural products of the Cauca Department-Colombia
DOI:
https://doi.org/10.15446/rcciquifa.v48n3.84992Palavras-chave:
Cromatografía líquida de alta resolución (CLAR), espectrometría de masas (EM), delta 9 tetrahidrocannabinol (Δ9 THC), cannabinol (CBN), cannabigerol (CBG), cannabidiol (CBD) (es)High performance liquid chromatography (HPLC), mass spectrometry (MS), delta 9 tetrahydrocannabinol (Δ9 THC), cannabinol (CBN), cannabigerol (CBG), cannabidiol (CBD) (en)
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Actualmente, hay un creciente interés por el estudio de Cannabis sativa y sus componentes ya que se le atribuye propiedades terapéuticas en el tratamiento de enfermedades. En Colombia y específicamente en el departamento del Cauca se comercializan productos de cannabis tanto para fines no medicinales como terapéuticos. En consecuencia, es necesario el análisis de estos productos de manera que se pueda conocer la composición de los mismos y el posible efecto que pueda tener sobre la salud.
El análisis de los componentes de estos productos se llevó a cabo empleando la cromatografía líquida de alta resolución (CLAR) y espectrometría de masas (EM), de tal manera que permitieron la identificación de las principales especies cannabinoides; Δ9tetrahidrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG). La separación de los analitos se llevó a cabo mediante la implementación de una columna analítica C18 de fase reversa, elución isocrática 1 mL/min, presión del sistema 800 PSI, una mezcla de acetonitrilo ACN y buffer fosfato (KHPO4) en relación 65/35 como fase móvil, volumen de inyección de 10 µL, un tiempo de análisis de 15 min, y detección a 220 nm.
Cannabis sativa has now experienced an increasing interest in the study of its components since it is attributed therapeutic properties in the treatment of diseases. In Colombia and specifically in the Cauca Department, Cannabis products are marketed both for non-medicinal and therapeutic purposes. Consequently, it is necessary to analyze these products in such a way that the composition of the products and their possible effect on health can be known. The analysis of the components of these products was carried out using high performance liquid chromatography (HPLC) and mass spectrometry (MS), in such a way that they allowed the identification of the main cannabinoid species; Δ 9tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG). The separation of the analytes was carried out by means of the implementation of a reverse phase C18 analytical column, isocratic elution 1 mL/min, system pressure 800 PSI, a mixture of acetonitrile ACN and phosphate buffer (KHPO4) in relation 65/35 as mobile phase, injection volume of 10 μL, analysis time of 15 min, and detection at 220 nm.
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1. Paola Andrea Arango Romero, Jhan Carlos Delgado, Sandra Marcela Muñoz, Luis Llanos, Ricardo Benítez. (2024). Actividad antioxidante de variedades de cannabis del Norte del Cauca, Colombia. Ciencia en Desarrollo, 15(1), p.178. https://doi.org/10.19053/01217488.v15.n1.2024.15214.
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