Publicado

2020-05-01

Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil

Plantas de tratamiento de aguas residuales domésticas como fuentes de Staphylococcus aureus resistente a macrólidos-lincosamida-estreptogramina B- y penicilina en Brasil

Estações de tratamento de águas residuais domésticas como fontes de Staphylococcus aureus resistente a macrolídeo-lincosamida-estreptograma e B- e penicilina no Brasil

DOI:

https://doi.org/10.15446/rcciquifa.v49n2.88854

Palavras-chave:

Antibiotic resistance, D-test, penicillin zone-edge test, quinolones, Staphylococcus aureus, water environment (en)
Resistencia a antibióticos, prueba D, prueba de borde de zona de penicilina, quinolonas, Staphylococcus aureus, ambiente acuático (es)
Resistência a antibióticos, D-teste, teste da borda da zona da penicilina, quinolonas, Staphylococcus aureus, ambiente aquático (pt)

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Autores

  • Sílvia Letícia Oliveira Toledo Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais
  • Renata Michelle Silveira Silva Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais
  • Isabella Cristina Rodrigues dos Santos Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais
  • William Gustavo Lima Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais
  • Leticia Gonçalves Rodrigues Ferreira Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais
  • Magna Cristina Paiva Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, Minas Gerais

Staphylococcus aureus is one of the main bacteria that affect human health. Its reduced susceptibility to beta-lactam antibiotics has driven the clinical use of macrolides and lincosamides. However, the presence of macrolide-lincosamide-streptogramin B (MLSB)-resistant S. aureus strains is increasingly common. Wastewater treatment plants (WWTPs) are the main anthropogenic source of resistance determinants. However, few studies have assessed the importance of this environment on the dissemination of MLSB-resistant S. aureus strains. Thus, we aimed to evaluate the impact of a domestic WWTP on the resistance to MLSB and penicillin in S. aureus in southeast Brazil. Of the 35 isolates tested, 40.6% were resistant to penicillin. Resistance to erythromycin (8.6%) and quinolones (2.8%) was less common. Despite the low rate of resistance to clindamycin (2.8%), many isolates showed reduced susceptibility to this antibiotic (57.1%). Regarding the resistance phenotypes of staphylococci isolates, inducible MLSB resistance (D-test positive) was found in two isolates. In addition, 27 S. aureus isolates showed the ability to produce penicillinase. In this article, we report for the first time the importance of WWTPs in the dissemination of MSLB resistance among S. aureus from southeast Brazil.

Staphylococcus aureus es una de las principales bacterias que afectan la salud humana. Su susceptibilidad reducida a los antibióticos betalactámicos ha impulsado el uso clínico de macrólidos y lincosamidas. Sin embargo, la presencia de cepas resistentes a macrólido-lincosamida-estreptogramina B (MLSB) de S. aureus es cada vez más común. Las plantas de tratamiento de aguas residuales (PTAR) son la principal fuente antropogénica de determinantes de resistencia. Sin embargo, pocos estudios han evaluado la importancia de este entorno en la diseminación de cepas de S. aureus resistentes a MLSB. Nuestro objetivo fue evaluar el impacto de una PTAR doméstica en MLSB y la resistencia a la penicilina en S. aureus en el sureste de Brasil. De los 35 aislamientos analizados, el 40,6% eran resistentes a la penicilina. La resistencia a la eritromicina (8,6%) y quinolonas (2,8%) fue menos común. A pesar de la baja tasa de resistencia a la clindamicina (2,8%), muchos aislamientos mostraron sensibilidad reducida a este antibiótico (57,1%). Con respecto a los fenotipos de resistencia de los aislamientos de estafilococos, la resistencia inducible a MLSB (prueba D positiva) se encontró en dos aislamientos. Además, 27 aislamientos de S. aureus mostraron la capacidad de producir penicilinasa. En este artículo informamos, por primera vez, la importancia de las PTAR en la difusión de la resistencia a MSLB entre S. aureus del sureste de Brasil.

O Staphylococcus aureus é uma das principais bactérias que afetam a saúde humana. Sua reduzida suscetibilidade aos antibióticos beta-lactâmicos tem impulsionado o uso clínico de macrolídeos e lincosamidas. No entanto, a presença de cepas de S. aureus resistentes a macrolídeo-lincosamida-estreptogramina B (MLSB) é cada vez mais comum. As estações de tratamento de esgoto (ETEs) são a principal fonte antropogênica de determinantes de resistência. No entanto, poucos estudos avaliaram a importância desse ambiente na disseminação de cepas de S. aureus resistentes ao MLSB. Assim, nosso objetivo foi avaliar o impacto de uma ETE doméstico na resistência ao MLSB e à penicilina em S. aureus no sudeste do Brasil. Dos 35 isolados testados, 40,6% eram resistentes à penicilina. Resistência à eritromicina (8,6%) e quinolonas (2,8%) foi menos comum. Apesar da baixa taxa de resistência à clindamicina (2,8%), muitos isolados apresentaram sensibilidade reduzida a esse antibiótico (57,1%). Em relação aos fenótipos de resistência dos isolados de estafilococos, a resistência induzível ao MLSB (D-teste positivo) foi encontrada em dois isolados. Além disso, 27 isolados de S. aureus mostraram a capacidade de produzir penicilinase. Neste artigo relatamos pela primeira vez a importância das ETEs na disseminação da resistência do MSLB entre S. aureus do sudeste do Brasil.

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Como Citar

APA

Oliveira Toledo, S. L., Silveira Silva, R. M., Rodrigues dos Santos, I. C., Lima, W. G., Rodrigues Ferreira, L. G. e Paiva, M. C. (2020). Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). https://doi.org/10.15446/rcciquifa.v49n2.88854

ACM

[1]
Oliveira Toledo, S.L., Silveira Silva, R.M., Rodrigues dos Santos, I.C., Lima, W.G., Rodrigues Ferreira, L.G. e Paiva, M.C. 2020. Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas. 49, 2 (maio 2020). DOI:https://doi.org/10.15446/rcciquifa.v49n2.88854.

ACS

(1)
Oliveira Toledo, S. L.; Silveira Silva, R. M.; Rodrigues dos Santos, I. C.; Lima, W. G.; Rodrigues Ferreira, L. G.; Paiva, M. C. Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil. Rev. Colomb. Cienc. Quím. Farm. 2020, 49.

ABNT

OLIVEIRA TOLEDO, S. L.; SILVEIRA SILVA, R. M.; RODRIGUES DOS SANTOS, I. C.; LIMA, W. G.; RODRIGUES FERREIRA, L. G.; PAIVA, M. C. Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 49, n. 2, 2020. DOI: 10.15446/rcciquifa.v49n2.88854. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/88854. Acesso em: 16 jul. 2024.

Chicago

Oliveira Toledo, Sílvia Letícia, Renata Michelle Silveira Silva, Isabella Cristina Rodrigues dos Santos, William Gustavo Lima, Leticia Gonçalves Rodrigues Ferreira, e Magna Cristina Paiva. 2020. “Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil”. Revista Colombiana De Ciencias Químico-Farmacéuticas 49 (2). https://doi.org/10.15446/rcciquifa.v49n2.88854.

Harvard

Oliveira Toledo, S. L., Silveira Silva, R. M., Rodrigues dos Santos, I. C., Lima, W. G., Rodrigues Ferreira, L. G. e Paiva, M. C. (2020) “Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil”, Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). doi: 10.15446/rcciquifa.v49n2.88854.

IEEE

[1]
S. L. Oliveira Toledo, R. M. Silveira Silva, I. C. Rodrigues dos Santos, W. G. Lima, L. G. Rodrigues Ferreira, e M. C. Paiva, “Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil”, Rev. Colomb. Cienc. Quím. Farm., vol. 49, nº 2, maio 2020.

MLA

Oliveira Toledo, S. L., R. M. Silveira Silva, I. C. Rodrigues dos Santos, W. G. Lima, L. G. Rodrigues Ferreira, e M. C. Paiva. “Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil”. Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 49, nº 2, maio de 2020, doi:10.15446/rcciquifa.v49n2.88854.

Turabian

Oliveira Toledo, Sílvia Letícia, Renata Michelle Silveira Silva, Isabella Cristina Rodrigues dos Santos, William Gustavo Lima, Leticia Gonçalves Rodrigues Ferreira, e Magna Cristina Paiva. “Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil”. Revista Colombiana de Ciencias Químico-Farmacéuticas 49, no. 2 (maio 1, 2020). Acessado julho 16, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/88854.

Vancouver

1.
Oliveira Toledo SL, Silveira Silva RM, Rodrigues dos Santos IC, Lima WG, Rodrigues Ferreira LG, Paiva MC. Domestic wastewater treatment plants as sources of macrolide-lincosamide-streptogramin B- and penicillin-resistant Staphylococcus aureus in Brazil. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 1º de maio de 2020 [citado 16º de julho de 2024];49(2). Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/88854

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1. María P. C. Mora-Gamboa, Sandra M. Rincón-Gamboa, Leidy D. Ardila-Leal, Raúl A. Poutou-Piñales, Aura M. Pedroza-Rodríguez, Balkys E. Quevedo-Hidalgo. (2022). Impact of Antibiotics as Waste, Physical, Chemical, and Enzymatical Degradation: Use of Laccases. Molecules, 27(14), p.4436. https://doi.org/10.3390/molecules27144436.

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