Published

2023-01-01

Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia

Efecto del algodón Bt (Cry1Ac y Cry2Ab) y no Bt en la variación temporal de A. grandis y representantes del complejo Spodoptera en Tolima, Colombia

DOI:

https://doi.org/10.15446/rfnam.v76n1.100904

Keywords:

Pest resistance, Population density, Transgenics plants (en)
Resistencia a plagas, Densidad de población, Plantas transgénicas (es)

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Transgenic cotton plants (Bacillus thuringiensis Berliner (Bt)) has significant influenced the integrated pest management around the world. In Colombia, Anthonomus grandis Boheman and Spodoptera complex are currently considered the main pests in cotton crops. Therefore, this study evaluated the effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the population fluctuation during two years in Tolima region. A Pearson correlation matrix was carried out between the pest variables and yield, while climatic variables and insect populations were correlated in four phenological stages with Spearman rank correlations. Additionally, a factor analysis for mixed data was performed in order to compare the effect of genotypes on the population fluctuation of the insects. For A. grandis, no differences in their populations were presented. However, in yield non-Bt cotton plants showed a higher inverse correlation with the perforated bolls compared to Bt cotton. In relation to the Spodoptera complex, the Bt genotype had 67.4% fewer larvae compared to non-Bt cotton. Statistically significant differences were presented. However, there was not a total absence of the pest during the entire crop cycle. These results suggest that if refuge zones and pest management practices are not determined in the study area, Spodoptera complex could generate resistance to genetically modified plants.

Las plantas de algodón transgénicas (Bacillus thuringiensis Berliner (Bt)) han influido significativamente en el manejo integrado de plagas en todo el mundo. En Colombia, Anthonomus grandis Boheman y el complejo Spodoptera son considerados actualmente las principales plagas en los cultivos de algodón. Por lo tanto, este estudio evaluó el efecto del algodón Bt (Cry1Ac y Cry2Ab) y no Bt en la fluctuación poblacional durante dos años en la región del Tolima. Se realizó una matriz de correlación de Pearson entre las variables plaga y rendimiento, mientras que las variables climáticas y las poblaciones de insectos fueron correlacionadas en cuatro estados fenológicos con la correlación de rangos de Spearman. Adicionalmente, se realizó un análisis factorial para datos mixtos con el fin de comparar el efecto de los genotipos sobre la fluctuación poblacional de los insectos. Para A. grandis, no se presentaron diferencias en sus poblaciones. Sin embargo, en rendimiento, las plantas de algodón no Bt mostraron una mayor correlación inversa con las cápsulas perforadas en comparación con el algodón Bt. Con relación al complejo Spodoptera, el genotipo Bt tuvo un 67,4% menos de larvas en comparación con el algodón no Bt. Se presentaron diferencias estadísticamente significativas. Sin embargo, no hubo ausencia total de la plaga durante todo el ciclo del cultivo. Estos resultados sugieren que, si en el área de estudio no se fijan zonas de refugio y prácticas de manejo de plagas, el complejo Spodoptera podría generar resistencia a plantas genéticamente modificadas.

References

Abd El-salam AME, Nemat AM and Magdy A. 2011. Potency of Bacillus thuringiensis and Bacillus subtilis against the cotton leafworm, Spodoptera littoralis (Bosid.) larvae. Archives of Phytopathology and Plant Protection 44(3): 204-215. https://doi.org/10.1080/03235400902952129

Bahar MH, Stanley J, Backhouse D, Mensah R, Del Socorro A and Gregg P. 2019. Survival of Helicoverpa armigera larvae on and Bt toxin expression in various parts of transgenic Bt cotton (Bollgard II) plants. Entomologia Experimentalis et Applicata 167(5): 415-423. https://doi.org/10.1111/eea.12792

Barros EM, Torres JB e Bueno AF. 2010. Oviposição, desenvolvimento e reprodução de Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) em diferentes hospedeiros de importância econômica. Neotropical Entomology 39(6): 996-1001. https://doi.org/10.1590/S1519-566X2010000600023

Bernardi D, Bernardi O, Horikoshi RJ, Salmeron E, Okuma DM, Farias JR, do Nascimento ARB and Omoto C. 2017. Selection and characterization of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to MON 89034× TC1507× NK603 maize technology. Crop protection 94: 64-68. https://doi.org/10.1016/j.cropro.2016.11.026

Britz C, Van Der Berg J and Du Plessis H. 2020. Susceptibility of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) to Bt cotton, expressing Cry1Ac and Cry2Ab toxins, in South Africa. African Entomology 28(1): 182-186. https://doi.org/10.4001/003.028.0182

Brookes G. 2020. Genetically modified (GM) crop use in Colombia: farm level economic and environmental contributions. GM Crops & Food 11(3): 140-153. https://doi.org/10.1080/21645698.2020.1715156

Carrière Y, Degain BA and Tabashnik BE. 2021. Effects of gene flow between Bt and non-Bt plants in a seed mixture of Cry1A.105 + Cry2Ab corn on performance of corn earworm in Arizona. Pest Management Science 77(4): 2106–2113. https://doi.org/10.1002/ps.6239

CONALGODON. 2020. Confederación Colombiana de algodón. Estadísticas. http://conalgodon.com/estadisticas/#sctabs-1631194323369 accessed: September 2021.

Da Silva FP, Bezerra APL e Da Silva AF. 2008. Oviposição e alimentação do bicudo, Anthonomus grandis Boheman, em linhagens mutantes de algodoeiro herbáceo de cultura de soca. Revista Ciência Agronômica 39(1): 85-89. http://www.ccarevista.ufc.br/seer/index.php/ccarevista/article/view/28/27

De Oliveira RS, Oliveira-Neto OB, Moura HF, De Macedo LL, Arraes F, Lucena WA, Lourenço-Tessutti IT, de Deus Barbosa AA, da Silva MCM and Grossi De Sa MF. 2016. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). Frontiers in Plant Science 7(165): 1-11. https://doi.org/10.3389/fpls.2016.00165

Gomes ES, Santos V and Ávila CJ. 2017. Biology and fertility life table of Helicoverpa armigera (Lepidoptera: Noctuidae) in different hosts. Entomological Science 20(1): 419-426. https://doi.org/10.1111/ens.12267

Grigolli JFJ, Aparecido de Souaza L, Felisbino-Fraga D, Funichello M and Busoli AG. 2013. Within plant distribution of Anthonomus grandis (Coleoptera: Curculionidae) feeding and oviposition damages in cotton cultivars. Ciência e Agrotecnologia UFLA 37(1): 78-84. https://doi.org/10.1590/S1413-70542013000100009

Huang F. 2021. Resistance of the fall armyworm, Spodoptera frugiperda, to transgenic Bacillus thuringiensis Cry1F corn in the Americas: lessons and implications for Bt corn IRM in China. Insect Science 28 (3): 574–589. https://doi.org/10.1111/1744-7917.12826

James C. 2015. 20th Anniversary (1996 to 2015) of the Global Status of Commercialized Biotech/GM Crops: 2015. ISAAA Briefs No. 51. Ithaca, NY. https://www.isaaa.org/resources/publications/briefs/51/

Jaramillo-Barrios CI, Barragan E and Monje-Andrade B. 2019. Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) cause significant damage to genetically modified corn crops. Revista Facultad Nacional de Agronomía Medellín 72(3): 8953-8962. https://doi.org/10.15446/rfnam.v72n3.75730

Jin Z, Yu W, Zhao H, Xian X, Jing K, Yang N Lu X and Liu W. 2022. Potential global distribution of invasive alien species, Anthonomus grandis Boheman, under current and future climate using optimal MaxEnt Model. Agriculture 12(11): 1-14. https://doi.org/10.3390/agriculture12111759

Khakwani K, Cengiz R, Naseer S, Asif M and Sarwar G. 2022. Cotton pink bollworm (Pectinophora gossypiella) management with the goal of eradication from the cotton producing countries of the world. Applied Ecology and Environmental Research 20(2): 1199– 1213. https://doi.org/10.15666/aeer/2002_11991213

Knight K, Head G and Rogers J. 2016. Relationships between Cry1Ac and Cry2Ab protein expression in field-grown Bollgard II® cotton and efficacy against Helicoverpa armigera and Helicoverpa punctigera (Lepidoptera: Noctuidae). Crop Protection 79(1): 150– 158. https://doi.org/10.1016/j.cropro.2015.10.013

Liu L, Gao M, Yang S, Liu S, Wu Y, Carrière Y and Yang Y. 2017. Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single‐toxin and pyramided cotton in cotton bollworm from China. Evolutionary Applications 10(2): 170:179. https://doi.org/10.1111/eva.12438

Meissle M, and Romeis J. 2018. Transfer of Cry1Ac and Cry2Ab proteins from genetically engineered Bt cotton to herbivores and predators. Insect Science 25(5): 823–832. https://doi.org/10.1111/1744-7917.12468

Nava-Camberos U, Ávila-Rodríguez V, Maltos-Buendía J, García-Hernández JL y Martínez-Carrillo JL. 2018. Densidades y daños de insectos plaga en algodonero convencional y Bt en la Comarca Lagunera, México. Southwestern Entomologist 43(4): 985-993. https://doi.org/10.3958/059.043.0415

Ñañez LC. 2012. Manejo fitosanitario del cultivo del algodón (Gossypium hirsutum). Disponible en: https://www.ica.gov.co/getattachment/a223d007-d6e6-4df0-a7fc-b0150cb6bbbb/Manejo-fitosanitario-del-cultivo-de-algodon.aspx ICA, Bogotá D. C. 43p.

Oliveira AA, Araújo TA, Showler AT, Araújo AC, Almeida IS, Aguiar RS, Miranda JE, Fernandes FL and Bastos CS. 2022. Spatio‐ temporal distribution of Anthonomus grandis grandis Boh. in tropical cotton fields. Pest Management Science 78(6) 2492-2501. https://doi.org/10.1002/ps.6880

Pascua LT, and Pascua ME. 2002. The distribution and movement of cotton bollworm, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) Larvae on Cotton. Philippine Journal of Science 131(2): 91-98.

Osorio-Almanza L, Burbano-Figueroa O y Martinez-Reina A. 2018. Factibilidad técnica de variedades de algodón expresando proteínas Cry tóxicas contra Anthonomus grandis en el Valle del Sinú, Colombia. Ciencia y Agricultura 15(2): 47-60. https://doi.org/10.19053/01228420.v15.n2.2018.8395

R Core Team. 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna: R Foundation for Statistical Computing.

Ribeiro EB, Castellani MA, Da Silva CA, Melo TL, Dos SantosSilva G, Do Vale W e Santos A. 2015. Métodos de destruição de restos de cultura do algodoeiro e sobrevivência do bicudo. Pesquisa Agropecuaria Brasileira 50(11): 993–998. http://dx.doi.org/10.1590/S0100-204X2015001100001

Ribeiro TP, Arraes FBM, Lourenço‐Tessutti IT, Silva MS, Lisei‐ de‐Sá ME, Lucena WA, de Macedo LLP, Lima JM, Santos Amorim RM, Artico S, Alvez-Ferreira M, Mattar Silva MC and Grossi‐de‐Sa MF. 2017. Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil. Plant Biotechnology Journal 15(8): 997-1009. https://doi.org/10.1111/pbi.12694

Salvador R, Príncipi D, Berretta M, Fernández P, Paniego N, Sciocco A and Hopp E. 2014. Transcriptomic survey of the midgut of Anthonomus grandis (Coleoptera: Curculionidae). Journal of Insect Science 14(1): 219-227. https://doi.org/10.1093/jisesa/ieu081

Salvador R, Niz JM, Nakaya PA, Pedarros A and Hopp HE. 2021. Midgut genes knockdown by oral dsRNA administration produces a lethal effect on cotton boll weevil. Neotropical Entomology 50(1): 121-128. https://doi.org/10.1007/s13744-020-00819-1

Santos-Amaya OF, Restrepo OD, Argüelles J y Garramuño EA. 2009. Evaluación del comportamiento del complejo Spodoptera con la introducción de algodón transgénico al Tolima, Colombia. Ciencia y Tecnología Agropecuaria 10(1): 24-32. DOI: https://doi.org/10.21930/rcta.vol10_num1_art:125

Sivasupramaniam S, Moar WJ, Ruschke LG, Osborn KA, Jiang C, Sebaugh JL, Brown GR, Shappley ZW, Oppenhuizen ME, Mullins JW and Greenplate JT. 2008. Toxicity and characterization of cotton expressing Bacillus thuringiensis Cry1Ac and Cry2Ab2 proteins for control of lepidopteran pests. Journal of Economic Entomology 101(2): 546-554. https://doi.org/10.1093/jee/101.2.546

Shad M, Yasmeen A, Azam S, Bakhsh A, Latif A, Shahid N, Din S, Sadaqat S, Rao AQ and Shahid, AA. 2022. Enhancing the resilience of transgenic cotton for insect resistance. Molecular Biology Reports 49(6):5315–5323. https://doi.org/10.1007/s11033-021-06972-z

Showalter AM, Heuberger S, Tabashnik BE and Carrière Y. 2009. A primer for using transgenic insecticidal cotton in developing countries. Journal of Insect Science 9(1): 22. https://doi.org/10.1673/031.009.2201

Showler AT, Greenberg SM, Scott Jr AW and Robinson JRC. 2005. Effects of planting dates on boll weevils (Coleoptera: Curculionidae) and cotton fruit in the subtropics. Journal of Economic Entomology 98(3): 796-804. https://doi.org/10.1603/0022-0493-98.3.796

Silva JB, Silva-Torres CSA, Moraes MCB, Torres JB, Laumann RA and Borges M. 2015. Interaction of Anthonomus grandis and cotton genotypes: Biological and behavioral responses. Entomologia Experimentalis et Applicata 156(3): 238-253. https://doi.org/10.1111/eea.12326

Sorenson CE and Stevens G. 2019. The boll weevil in Missouri: history, biology and management. In: Extrension University of Missouri, Available at: https://mospace.umsystem.edu/xmlui/bitstream/handle/10355/83965/G04255.pdf?sequence=1&isAllowed=y. 5p.

Valencia-Cataño SJ, Rodríguez Charlarca J and Mesa Cobo NC. 2014. Effect of varieties of cotton GM on Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae. Acta Agronómica 63(1): 63-70. https://doi.org/10.15446/acag.v63n1.38356

Yang F, Kerns DL, Leonard BR, Oyediran I, Burd T, Niu Y and Huang F. 2015. Performance of Agrisure® VipteraTM 3111 corn against Helicoverpa zea (Lepidoptera: Noctuidae) in seed mixed plantings. Crop Protection 69(1): 77-82. https://doi.org/10.1016/j.cropro.2014.12.002

Yang F, Kerns DL, Head G, Brown S and Huang F. 2017. Susceptibility of Cry1F-maize resistant, heterozygous, and susceptible Spodoptera frugiperda to Bt proteins used in the transgenic cotton. Crop Protection 98(1): 128–135. https://doi.org/10.1016/j.cropro.2017.03.023

Yang F, Head GP, Price PA, Santiago González JC and Kerns DL. 2020. Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae). Pest Management Science 76(11): 3676–3684. https://doi.org/10.1002/ps.5916

Zakir A, Khallaf MA, Hansson BS, Witzgall P and Anderson P. 2017. Herbivore-induced changes in cotton modulates reproductive behavior in the moth Spodoptera littoralis. Frontiers in Ecology and Evolution 5(49): 1-10. https://doi.org/10.3389/fevo.2017.00049

How to Cite

APA

Jaramillo-Barrios, C. I., Cruz-Castiblanco, G. N. & Monje-Andrade, B. (2023). Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia. Revista Facultad Nacional de Agronomía Medellín, 76(1), 10157–10166. https://doi.org/10.15446/rfnam.v76n1.100904

ACM

[1]
Jaramillo-Barrios, C.I., Cruz-Castiblanco, G.N. and Monje-Andrade, B. 2023. Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia. Revista Facultad Nacional de Agronomía Medellín. 76, 1 (Jan. 2023), 10157–10166. DOI:https://doi.org/10.15446/rfnam.v76n1.100904.

ACS

(1)
Jaramillo-Barrios, C. I.; Cruz-Castiblanco, G. N.; Monje-Andrade, B. Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia. Rev. Fac. Nac. Agron. Medellín 2023, 76, 10157-10166.

ABNT

JARAMILLO-BARRIOS, C. I.; CRUZ-CASTIBLANCO, G. N.; MONJE-ANDRADE, B. Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia. Revista Facultad Nacional de Agronomía Medellín, [S. l.], v. 76, n. 1, p. 10157–10166, 2023. DOI: 10.15446/rfnam.v76n1.100904. Disponível em: https://revistas.unal.edu.co/index.php/refame/article/view/100904. Acesso em: 18 mar. 2026.

Chicago

Jaramillo-Barrios, Camilo Ignacio, Ginna Natalia Cruz-Castiblanco, and Buenaventura Monje-Andrade. 2023. “Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia”. Revista Facultad Nacional De Agronomía Medellín 76 (1):10157-66. https://doi.org/10.15446/rfnam.v76n1.100904.

Harvard

Jaramillo-Barrios, C. I., Cruz-Castiblanco, G. N. and Monje-Andrade, B. (2023) “Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia”, Revista Facultad Nacional de Agronomía Medellín, 76(1), pp. 10157–10166. doi: 10.15446/rfnam.v76n1.100904.

IEEE

[1]
C. I. Jaramillo-Barrios, G. N. Cruz-Castiblanco, and B. Monje-Andrade, “Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia”, Rev. Fac. Nac. Agron. Medellín, vol. 76, no. 1, pp. 10157–10166, Jan. 2023.

MLA

Jaramillo-Barrios, C. I., G. N. Cruz-Castiblanco, and B. Monje-Andrade. “Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia”. Revista Facultad Nacional de Agronomía Medellín, vol. 76, no. 1, Jan. 2023, pp. 10157-66, doi:10.15446/rfnam.v76n1.100904.

Turabian

Jaramillo-Barrios, Camilo Ignacio, Ginna Natalia Cruz-Castiblanco, and Buenaventura Monje-Andrade. “Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia”. Revista Facultad Nacional de Agronomía Medellín 76, no. 1 (January 1, 2023): 10157–10166. Accessed March 18, 2026. https://revistas.unal.edu.co/index.php/refame/article/view/100904.

Vancouver

1.
Jaramillo-Barrios CI, Cruz-Castiblanco GN, Monje-Andrade B. Effect of Bt (Cry1Ac and Cry2Ab) and non-Bt cotton on the temporal variation of A. grandis and representatives of the Spodoptera complex in Tolima, Colombia. Rev. Fac. Nac. Agron. Medellín [Internet]. 2023 Jan. 1 [cited 2026 Mar. 18];76(1):10157-66. Available from: https://revistas.unal.edu.co/index.php/refame/article/view/100904

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1. Raul Narciso C. Guedes, Leonardo M. Turchen, Ran Wang, Evgenios Agathokleous. (2024). Bioinsecticides and non-target pest species. Current Opinion in Environmental Science & Health, 41, p.100570. https://doi.org/10.1016/j.coesh.2024.100570.

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