Published

2008-07-01

Determinación de la dl50 y tl50 de extractos etanólicos de suspensiones celulares de Azadirachta indica sobre Spodoptera frugiperda.

Keywords:

Cogollero del maíz, Metabolitos secundarios, Modelos de elección discreta (probit), Neem, Suspensión celular. (es)

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Authors

  • Paula Andrea Trujillo Ruiz
  • Leidy Natalia Zapata Restrepo
  • Rodrigo Alberto Hoyos Sánchez
  • Francisco Cristóbal Yepes Rodríguez
  • Jacqueline Capataz Tafur
  • Fernando Orozco Sánchez
La obtención in vitro de metabolitos secundarios de neem surge como una alternativa para la posible producción industrial de biocidas. Para la búsqueda de propuestas dirigidas al control de Spodoptera frugiperda, se evaluó la eficiencia de extractos etanólicos de suspensiones celulares de Azadirachta indica para determinar la DL50 y el TL50 sobre la fase larval del segundo ínstar (L2). Se probaron cuatro concentraciones de los extractos (2.500, 5.000, 10.000 y 30.000 ppm) y el testigo (alcohol 96%) para determinar la mortalidad en el tiempo (0, 12, 18, 25, 40 h). Se determinó una DL50 a las 12 h de 2.256 ppm (R2 = 88,54%), a las 18 h de 3.928 ppm (R2 = 90,3 %), a las 25 h de 2.818 ppm (R2 = 90,3 %) y a las 40 h de 1.064 ppm (R2 =90,3 %). Las dosis de 5.000 ppm, 10.000 ppm y 30.000 ppm, no presentaron diferencia estadística en su acción letal, pero fueron efectivas en el control del insecto.

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