Association between FSHR polymorphism with productive and reproductive traits in Antioquia Holstein cattle

Published

2016-01-01

Asociación entre un polimorfismo de FSHR con características productivas y reproductivas en el ganado Holstein de Antioquia

Keywords:

bovine, FSH, PCR-RFLP, productive traits, reproductive traits, SNP (en)
Bovino, FSH, PCR-RFLP, Parámetros productivos, Parámetros reproductivos, SNP (es)

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Authors

Stephania Madrid Gaviria Universidad Nacional de Colombia
Albeiro López Herrera Universidad Nacional de Colombia
José Julián Echeverri Zuluaga Universidad Nacional de Colombia

Abstract (en)
Abstract (es)

Because FSH and its receptor play a fundamental role in reproduction, the objective of this research was determining the effect of the A-320T polymorphism in productive and reproductive traits in Antioquia Holstein cows. The PCR-RFLP was used to amplify a segment of 970 bp of the bovine follicle stimulating hormone receptor gene (FSHR) which was digested with the restriction enzyme TaqI. The effect of the FSHR genotypes on productive and reproductive traits was determinate by a Mixed Linear Model and Tukey Test was used to establish significant differences between means for the three genotypes. The effect of allelic substitution was studied through a linear regression model where the genotypes AA, AT and TT were transformed into a quantitative scale of 0, 1 and 2, respectively according to the number of possessed T alleles. In Antioquia Holstein cattle the most common genotype was the AT (0.485) followed by TT (0.417) and AA (0.096) genotypes. Allele frequencies were 0.339 for A and 0.660 for T, respectively. The FSHR genotypes did not exert a significant effect on the principal productive parameters, except for fat percentage (P<0.01) where the TT individuals presented the highest percent. Results showed that T allele seems to improve the solids in milk while A allele improves dairy yield. The reproductive parameters were not affected by this SNP but AT animals showed a higher number of services per conception. Further studies are required to determine whether this SNP may be used as a molecular marker.

Debido a que la FSH y su receptor cumplen un rol fundamental para la reproducción, el objetivo de este estudio fue determinar el efecto del polimorfismo A-320T en parámetros productivos y reproductivos en el ganado Holstein de Antioquia. Se utilizó la PCR-RFLP para amplificar un segmento de 970 pb del gen que codifica para el receptor de la hormona folículo estimulante (FSHR) que se digirió con la endonucleasa TaqI. El efecto de los genotipos de FSHR en los parámetros productivos y reproductivos se determinó mediante un Modelo Linear Mixto y se empleó la prueba de Tukey para establecer diferencias significativas entre las medias de los genotipos. El efecto de sustitución alélica se estudió mediante un modelo de regresión lineal donde los genotipos AA, AT y TT se transformaron a una escala de 0, 1 y 2 de acuerdo con el número de alelos T poseídos. Para el ganado Holstein de Antioquia el genotipo más común fue el AT (0,485) seguido por el TT (0,0417) y el AA (0,096). Las frecuencias alélicas fueron 0,339 y 0,660 para A y T. Los genotipos de FSHR no presentaron un efecto significativo en los parámetros productivos, excepto para el porcentaje de grasa (P<0.01) donde los individuos TT presentaron el mayor porcentaje. Los resultados mostraron que el alelo T parece mejorar el contenido de sólidos en leche, mientras que el alelo A mejora la producción de leche. Los parámetros reproductivos no se vieron afectados por este SNP pero los animales AT mostraron un mayor número de servicios por concepción. Se requieren más estudios para determinar si este SNP puede ser usado como marcador molecular.

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