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Antioxidant activity and GC-MS profile of Conyza bonariensis L. leaves extract and fractions
Actividad antioxidante y perfil CG-EM del extracto y fracciones de hojas de Conyza bonariensis L.
DOI:
https://doi.org/10.15446/rfnam.v73n3.81452Keywords:
bioactive compounds, DPPH, flavonoid, fractionation, FRAP, polyphenol (en)DPPH, flavonóide, fracionamento, FRAP, polifenol (pt)
compuestos bioactivos, DPPH, flavonoide, fractionamiento, FRAP, polifenoles (es)
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The 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power (FRAP), and semivolatile compounds of Conyza bonariensis L. leave extract and fractions are discussed. A methanolic crude extract was obtained through maceration, and subsequently, n-hexane, chloroform, and ethyl acetate fractions were collected using a solvent-solvent partition. Total phenols, flavonoids, and antioxidant activity assays were performed in an ultraviolet-visible (UV-Vis) spectrophotometer, and the results were expressed as Gallic Acid, Quercetin, and Trolox equivalents respectively. The findings achieved indicate that ethyl acetate fraction showed the highest DPPH radical scavenging capacity (90.69±3.16%) at 500 µg mL-1, and reduced the ferric tripyridyltriazine complex (Fe3+-TPTZ) with values between 19.68 and 2,355.37 mg Trolox equivalent (TE) g-1. It was identified 28 phytoconstituents through Gas chromatography-mass spectrometry (GC-MS). The scavenging activity of ethyl acetate fraction could be correlated mostly to the presence of eugenol, trans-isoeugenol, lucenin-2, methyl salicylate, and syringic acid. This study reveals that the ethyl acetate fraction could be used as a good source of antioxidants for health benefits.
El 2,2-difenil-1-picrilhidrazil (DPPH), el poder reductor férrico (FRAP) y los compuestos semivolátiles del extracto y fracciones de Conyza bonariensis L. son discutidos. Se obtuvo un extracto crudo en metanol mediante maceración y posteriormente se recogieron fracciones de n-hexano, cloroformo y acetato de etilo usando partición disolvente-disolvente. Los ensayos de fenoles totales, flavonoides y actividad antioxidante se efectuaron en un espectrofotómetro (UV-Vis) y los resultados se expresaron como equivalentes de Ácido gálico, Quercetina y Trolox, respectivamente. Los resultados indican que la fracción en acetato de etilo mostró la mayor capacidad de eliminación del radical DPPH (90.69±3.16%) a 500 µg mL-1 y redujo el complejo de tripiridiltriazina férrico (Fe3+-TPTZ) con valores entre 19.68 y 2355.37 mg equivalente de Trolox (TE) g-1. Se identificaron 28 fitoconstituyentes a través del análisis de cromatografía de gases-espectrometría de masas (CG-EM). La actividad antioxidante presentada puede correlacionarse principalmente con la presencia de eugenol, trans-isoeugenol, lucenina-2, salicilato de metilo y ácido siríngico detectado. Este estudio muestra que la fracción de acetato de etilo podría ser utilizada como una buena fuente de antioxidantes para el beneficio de la salud humana.
References
Balladares C, Chóez-Guaranda I, García J, Sosa D, Pérez S, González JE, Viteri R, Barragán A, Quijano-Avilés M and Manzano P. 2016. Physicochemical characterization of Theobroma cacao L. sweatings in Ecuadorian coast. Emirates Journal of Food and Agriculture 28(10): 741-745. doi: 10.9755/ejfa.2016-02-187
Basile A, Sorbo S, López-Sáez JA, and Castaldo Cobianchi R. 2003. Effects of seven pure flavonoids from mosses on germination and growth of Tortula muralis HEDW. (Bryophyta) and Raphanus sativus L. (Magnoliophyta). Phytochemistry. 62(7): 1145–1151. doi: 10.1016/S0031-9422(02)00659-3
Ceylan R, Katanić J, Zengin G, Matić S, Aktumsek A, Boroja T, Stanić S, Mihailović V, Guler GO, Boga M and Yılmaz MA. 2016. Chemical and biological fingerprints of two Fabaceae species (Cytisopsis dorycniifolia and Ebenus hirsuta): Are they novel sources of natural agents for pharmaceutical and food formulations? Industrial Crops and Products 84: 254–262. doi: 10.1016/j.indcrop.2016.02.019
Choe E and Min DB. 2009. Mechanisms of Antioxidants in the Oxidation of Foods. Comprehensive Reviews in Food Science and Food Safety 8(4): 345–358. doi: 10.1111/j.1541-4337.2009.00085.x
Daur I. 2015. Chemical composition of selected Saudi medicinal plants. Arabian Journal of Chemistry 8(3): 329–332. doi: 10.1016/j.arabjc.2013.10.015
Diaz P, Jeong SC, Lee S, Khoo C and Koyyalamudi SR. 2012. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese medicine. 7(1): 26. doi: 10.1186/1749-8546-7-26
Ebada SS, Edrada RA, Lin W and Proksch P. 2008. Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates. Nature Protocols 3(12): 1820–1831. doi: 10.1038/nprot.2008.182
El Zalabani SM, Hetta MH and Ismail AS. 2012. Genetic Profiling, Chemical Characterization and Biological Evaluation of Two Conyza Species Growing in Egypt. Journal of Applied Pharmaceutical Science 2(11): 54–61. doi: 10.7324/JAPS.2012.21110
Figueroa L, Navarro L, Vera M and Petricevich V. 2015. Antioxidant activity, total phenolic and flavonoid contents, and cytotoxicity evaluation of Bougainvillea xbuttiana. International Journal of Pharmacy and Pharmaceutical Sciences 6(5): 497–502.
Giri GF, Danielli M, Marinelli RA and Spanevello RA. 2016. Cytotoxic effect of levoglucosenone and related derivatives against human hepatocarcinoma cell lines. Bioorganic & Medicinal Chemistry Letters 26(16): 3955–3957. doi: 10.1016/j.bmcl.2016.07.007
Hayet E, Maha M, Samia A, Ali MM, Souhir B, Abderaouf K, Mighri Z and Mahjoub A. 2009. Antibacterial, antioxidant and cytotoxic activities of extracts of Conyza canadensis (L.) Cronquist growing in Tunisia. Medicinal Chemistry Research 18(6): 447–454. doi: 10.1007/s00044-008-9141-0
Hoffmann-Bohm K, Lotter H, Seligmann O and Wagner H. 1992. Antihepatotoxic C-glycosylflavones from the leaves of Allophyllus edulis var. edulis and gracilis. Planta Medica 58(6): 544–548. doi: 10.1055/s-2006-961546
Kamdem-Boniface P and Pal A. 2013. Substantiation of the ethnopharmacological use of Conyza sumatrensis (Retz.) E.H.Walker in the treatment of malaria through in vivo evaluation in Plasmodium berghei infected mice. Journal of Ethnopharmacology 145(1): 373–377. doi: 10.1016/j.jep.2012.10.025
Kong LD, Abliz Z, Zhou CX, Li LJ, Cheng CHK and Tan RX. 2001. Glycosides and xanthine oxidase inhibitors from Conyza bonariensis. Phytochemistry 58(4): 645–651. doi: 10.1016/S0031-9422(01)00176-5
Krishnan R and Murugan K. 2014. Comparison of GC-MS analysis of phytochemicals in the ethanolic extracts of Marchantia linearis Lehm & Lindenb. and Marchantia polymorpha L. (Bryophyta). International Journal of Pharmaceutical Sciences and Research. 49(5): 1981–1987. doi: 10.13040/IJPSR.0975-8232.5(5).1981-87
Kumar S, Prahalathan P and Raja B. 2012. Syringic acid ameliorates l-NAME-induced hypertension by reducing oxidative stress. Naunyn-Schmiedeberg's Archives of Pharmacology 385(12): 1175–1184. doi: 10.1007/s00210-012-0802-7
Li H, Deng Z, Zhu H, Hu C, Liu R, Young JC and Tsao R. 2012. Highly pigmented vegetables: Anthocyanin compositions and their role in antioxidant activities. Food Research International 46(1): 250–259. doi: 10.1016/j.foodres.2011.12.014
Marjoni MR and Zulfisa A. 2017. Antioxidant Activity of Methanol Extract/Fractions of Senggani Leaves (Melastoma candidum D. Don). Pharmaceutica Analytica Acta 8(8). doi: 10.4172/2153-2435.1000557
Min B, McClung AM and Chen MH. 2011. Phytochemicals and Antioxidant Capacities in Rice Brans of Different Color. Journal of Food Science 76(1): C117–C126. doi: 10.1111/j.1750-3841.2010.01929.x
Mussin JE, Manzano P, Mangiaterra M and Giusiano G. 2017. Inhibitory activity of Conyza bonariensis (L.) Cronquist tincture against fungi and bacteria causing superficial infections. Revista Cubana de Plantas Medicinales 22(3).
Okumu MN, Vorster BJ and Reinhardt CF. 2019. Growth-stage and temperature influence glyphosate resistance in Conyza bonariensis (L.) Cronquist. South African Journal of Botany 121: 248–256. doi: 10.1016/j.sajb.2018.10.034
Rodríguez AT, Morales D and Ramírez MA. 2000. Efecto de extractos vegetales sobre el crecimiento in vitro de hongos fitopatógenos. Cultivos Tropicales. 21(2): 79–82.
Sacchi KL, Bisson LF and Adams DO. 2005. A review of the effect of winemaking techniques on phenolic extraction in red wines. American Journal of Enology and Viticulture 56(3): 197–206.
Saluk-Juszczak J, Pawlaczyk I, Olas B, Kołodziejczyk J, Ponczek M, Nowak P, Tsirigotis-Wołoszczak M, Wachowicz B and Gancarz R. 2010. The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins. International Journal of Biological Macromolecules 47(5): 700–705. doi: 10.1016/j.ijbiomac.2010.09.007
Santos PC, Santos VHM, Mecina GF, Andrade AR, Fegueiredo PA, Moraes VMO, Silva LP and Silva RMG. 2015. Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth. South African Journal of Botany 100: 114–121. doi: 10.1016/j.sajb.2015.05.013
Shahwar D, Raza MA, Saeed A, Riasat M, Chattha FI, Javaid M, Ullah S and Ullah S. 2012. Antioxidant potential of the extracts of Putranjiva roxburghii, Conyza bonariensis, Woodfordia fruiticosa and Senecio chrysanthemoids. African Journal of Biotechnology 11(18): 4288–4295. doi: 10.5897/AJB11.2564
Shoaib M, Shah I, Ali N, Adhikari A, Tahir MN, Shah SWA, Ishtiaq S, Khan J, Khan S and Umer MN. 2017. Sesquiterpene lactone! a promising antioxidant, anticancer and moderate antinociceptive agent from Artemisia macrocephala jacquem. BMC Complementary and Alternative Medicine 17(27): 1–11. doi: 10.1186/s12906-016-1517-y
Singh D and Chaudhuri PK. 2018. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L.). Industrial Crops and Products 118: 367–382. doi: 10.1016/j.indcrop.2018.03.048
Tennyson S, Balaraju K, Park K, Ravindran KJ, Eapen A and William SJ. 2012. In vitro antioxidant activity of Ageratum houstonianum Mill. (Asteraceae). Asian Pacific Journal of Tropical Disease 2 Suppl. 2: S712–S714. doi: 10.1016/S2222-1808(12)60249-7
Thabit RA, Cheng XR, Tang X, Sun J, Shi YH and Le GW. 2015. Antioxidant and antibacterial activities of extracts from Conyza bonariensis growing in Yemen. Pakistan journal of pharmaceutical sciences. 28(1): 129–134.
Waterhouse AL. 2002. Determination of Total Phenolics. Current Protocols in Food Analytical Chemistry 6(1):1-8. doi: 10.1002/0471142913.fai0101s06
Zambrano-Navea CL, Bastida F and González-Andújar JL. 2016. A cohort-based stochastic model of the population dynamic and long-term management of Conyza bonariensis in fruiting tree crops. Crop Protection 80(6): 15–20. doi: 10.1016/j.cropro.2015.10.023
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