Publicado

2022-02-04

In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates

Análisis in silico e in vitro de una nueva sustancia antifúngica potencial, 2-Bromo-N-fenilacetamida, contra cepas de candidiasis invasive

Análise in silico e in vitro de uma nova substância antifúngica potencial, 2-Bromo-N-fenilacetamida, contra isolados de candidíase invasiva

DOI:

https://doi.org/10.15446/rcciquifa.v50n3.100227

Palabras clave:

Candida albicans, Candida parapsilosis, 2-Bromo-N-phenylacetamide, antifungal (en)
Candida albicans, Candida parapsilosis, 2-bromo-N-fenilacetamida, antifúngico (es)
Candida albicans, Candida parapsilosis, 2-bromo-N-fenilacetamida, Antifúngico (pt)

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Autores/as

  • Romulo Pereira de Moura Sousa Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Daniele de Figuerêdo Silva Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Neuza Maria Cavalcante Oliveira Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Zélia Braz Vieira Da Silva Pontes Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Hermes Diniz Neto Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Laísa Vilar Cordeiro Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Pedro Thiago Ramalho de Figueiredo Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Rafael Farias de Oliveira Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Helivaldo Diógenes da Silva Souza Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Petrônio Filgueiras de Athayde Filho Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa
  • Edeltrudes de Oliveira Lima Federal University of Paraíba, Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, João Pessoa

Introduction: invasive candidiasis is related to high rates of morbidity and mortality. There are few classes of drugs available for the treatment of this type of infection and the index of resistant strains is increasing. Such circumstances highlight that the search for new pharmacotherapeutic alternatives is increasingly necessary. This study investigated 2-Bromo-N-phenylacetamide, a substance whose antifungal activity has not yet been reported. Objective: to evaluate its activity against invasive candidiasis isolates, by determining the minimum inhibitory and fungicide concentrations. Meth-odology: molecular docking was performed to investigate the possible mechanism of action of the substance. The substance was also associated with fluconazole, to assess the viability of the combination in clinical practice. The minimum inhibitory concen-trations ranged between 4 to 32 μg/mL, and it acts in a fungicidal way. Results: molec-ular docking suggests that 2-Bromo-N-phenylacetamide possibly acts on the fungal plasma membrane. And the association of 2-Bromo-N-phenylacetamide with flucon-azole against resistant strains showed an indifferent effect. Conclusion: further studies should be carried out to elucidate the potential of this substance, which may become a future drug candidate to treat invasive candidiasis and other fungal infections.

Introducción: la candidiasis invasiva está relacionada con altas tasas de morbilidad y mortalidad. Hay pocas clases de medicamentos disponibles para el tratamiento de este tipo de infección y el índice de cepas resistentes está aumentando. Tales circunstancias ponen de relieve que la búsqueda de nuevas alternativas farmacoterapéuticas es cada vez más necesaria. Este estudio investigó la 2-Bromo-N-fenilacetamida, una sustancia cuya actividad antifúngica aún no se ha informado. Objetivo: evaluar su actividad frente a aislados de candidiasis invasiva, mediante la determinación de las concentra-ciones mínimas inhibitorias y fungicidas. Metodología: se realizó un acoplamiento molecular para investigar el posible mecanismo de acción de la sustancia. La sustancia también se asoció con fluconazol, para evaluar la viabilidad de la combinación en la práctica clínica. Las concentraciones mínimas inhibidoras oscilaron entre 4 a 32 μg/mL y actúa de forma fungicida. Resultados: el acoplamiento molecular sugiere que la 2-Bromo-N-fenilacetamida posiblemente actúa sobre la membrana plasmática de los hongos. Y la asociación de 2-Bromo-N-fenilacetamida con fluconazol contra cepas resistentes mostró un efecto indiferente. Conclusión: deben realizarse más estudios para dilucidar el potencial de esta sustancia, que puede convertirse en un futuro candi-dato a fármaco para tratar la candidiasis invasiva y otras infecciones fúngicas.

Introducão:a candidíase invasiva está relacionada a altas taxas de morbidade e morta-lidade. Existem poucas classes de medicamentos disponíveis para o tratamento desse tipo de infecção e o índice de cepas resistentes está aumentando. Tais circunstâncias evidenciam que a busca por novas alternativas farmacoterapêuticas é cada vez mais necessária. Este estudo investigou a 2-Bromo-N-fenilacetamida, uma substância cuja atividade antifúngica ainda não foi relatada. Objetivo: avaliar sua atividade contra isolados de candidíase invasiva, por meio da determinação das concentrações mínimas inibitórias e fungicidas. Metodologia: o docking molecular foi realizado para inve-stigar o possível mecanismo de ação da substância. A substância também foi associada ao fluconazol, para avaliar a viabilidade da associação na prática clínica. As concen-trações inibitórias mínimas variaram entre 4 a 32 μg/Ml e atuam de forma fungicida. Resultados: o docking molecular sugere que a 2-Bromo-N-fenilacetamida possivel-mente atua na membrana plasmática do fungo. E a associação de 2-Bromo-N-fenilace-tamida com fluconazol contra cepas resistentes mostrou efeito indiferente. Conclusão: Novos estudos devem ser realizados para elucidar o potencial dessa substância, que pode se tornar uma futura droga candidata ao tratamento de candidíase invasiva e outras infecções fúngicas.

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Cómo citar

APA

de Moura Sousa, R. P., de Figuerêdo Silva, D., Cavalcante Oliveira, N. M., Vieira Da Silva Pontes, Z. B., Diniz Neto, H., Vilar Cordeiro, L., Ramalho de Figueiredo, P. T., Farias de Oliveira, R., da Silva Souza, H. D., de Athayde Filho, P. F. y de Oliveira Lima, E. (2022). In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates. Revista Colombiana de Ciencias Químico-Farmacéuticas, 50(3). https://doi.org/10.15446/rcciquifa.v50n3.100227

ACM

[1]
de Moura Sousa, R.P., de Figuerêdo Silva, D., Cavalcante Oliveira, N.M., Vieira Da Silva Pontes, Z.B., Diniz Neto, H., Vilar Cordeiro, L., Ramalho de Figueiredo, P.T., Farias de Oliveira, R., da Silva Souza, H.D., de Athayde Filho, P.F. y de Oliveira Lima, E. 2022. In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates. Revista Colombiana de Ciencias Químico-Farmacéuticas. 50, 3 (feb. 2022). DOI:https://doi.org/10.15446/rcciquifa.v50n3.100227.

ACS

(1)
de Moura Sousa, R. P.; de Figuerêdo Silva, D.; Cavalcante Oliveira, N. M.; Vieira Da Silva Pontes, Z. B.; Diniz Neto, H.; Vilar Cordeiro, L.; Ramalho de Figueiredo, P. T.; Farias de Oliveira, R.; da Silva Souza, H. D.; de Athayde Filho, P. F.; de Oliveira Lima, E. In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates. Rev. Colomb. Cienc. Quím. Farm. 2022, 50.

ABNT

DE MOURA SOUSA, R. P.; DE FIGUERÊDO SILVA, D.; CAVALCANTE OLIVEIRA, N. M.; VIEIRA DA SILVA PONTES, Z. B.; DINIZ NETO, H.; VILAR CORDEIRO, L.; RAMALHO DE FIGUEIREDO, P. T.; FARIAS DE OLIVEIRA, R.; DA SILVA SOUZA, H. D.; DE ATHAYDE FILHO, P. F.; DE OLIVEIRA LIMA, E. In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 50, n. 3, 2022. DOI: 10.15446/rcciquifa.v50n3.100227. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/100227. Acesso em: 4 ago. 2024.

Chicago

de Moura Sousa, Romulo Pereira, Daniele de Figuerêdo Silva, Neuza Maria Cavalcante Oliveira, Zélia Braz Vieira Da Silva Pontes, Hermes Diniz Neto, Laísa Vilar Cordeiro, Pedro Thiago Ramalho de Figueiredo, Rafael Farias de Oliveira, Helivaldo Diógenes da Silva Souza, Petrônio Filgueiras de Athayde Filho, y Edeltrudes de Oliveira Lima. 2022. «In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates». Revista Colombiana De Ciencias Químico-Farmacéuticas 50 (3). https://doi.org/10.15446/rcciquifa.v50n3.100227.

Harvard

de Moura Sousa, R. P., de Figuerêdo Silva, D., Cavalcante Oliveira, N. M., Vieira Da Silva Pontes, Z. B., Diniz Neto, H., Vilar Cordeiro, L., Ramalho de Figueiredo, P. T., Farias de Oliveira, R., da Silva Souza, H. D., de Athayde Filho, P. F. y de Oliveira Lima, E. (2022) «In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates», Revista Colombiana de Ciencias Químico-Farmacéuticas, 50(3). doi: 10.15446/rcciquifa.v50n3.100227.

IEEE

[1]
R. P. de Moura Sousa, «In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates», Rev. Colomb. Cienc. Quím. Farm., vol. 50, n.º 3, feb. 2022.

MLA

de Moura Sousa, R. P., D. de Figuerêdo Silva, N. M. Cavalcante Oliveira, Z. B. Vieira Da Silva Pontes, H. Diniz Neto, L. Vilar Cordeiro, P. T. Ramalho de Figueiredo, R. Farias de Oliveira, H. D. da Silva Souza, P. F. de Athayde Filho, y E. de Oliveira Lima. «In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 50, n.º 3, febrero de 2022, doi:10.15446/rcciquifa.v50n3.100227.

Turabian

de Moura Sousa, Romulo Pereira, Daniele de Figuerêdo Silva, Neuza Maria Cavalcante Oliveira, Zélia Braz Vieira Da Silva Pontes, Hermes Diniz Neto, Laísa Vilar Cordeiro, Pedro Thiago Ramalho de Figueiredo, Rafael Farias de Oliveira, Helivaldo Diógenes da Silva Souza, Petrônio Filgueiras de Athayde Filho, y Edeltrudes de Oliveira Lima. «In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates». Revista Colombiana de Ciencias Químico-Farmacéuticas 50, no. 3 (febrero 4, 2022). Accedido agosto 4, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/100227.

Vancouver

1.
de Moura Sousa RP, de Figuerêdo Silva D, Cavalcante Oliveira NM, Vieira Da Silva Pontes ZB, Diniz Neto H, Vilar Cordeiro L, Ramalho de Figueiredo PT, Farias de Oliveira R, da Silva Souza HD, de Athayde Filho PF, de Oliveira Lima E. In silico and in vitro analysis of a new potential antifungal substance, 2-Bromo-Nphenylacetamide, against invasive candidiasis isolates. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 4 de febrero de 2022 [citado 4 de agosto de 2024];50(3). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/100227

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