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Modulation of IL-6 in ameliorating arthritic conditions in rats by Rosa alba L flower extract and fraction
Modulación de IL-6 en la mejora de afecciones artríticas en ratas por extracto y fracción de flor de Rosa alba L
Modulação de IL-6 na melhora de condições artríticas em ratos por extrato e fração de flor de Rosa alba L
DOI:
https://doi.org/10.15446/rcciquifa.v52n1.103423Palabras clave:
Complete freund’s adjuvant, histopathology, HPTLC, interleukin, quercetin (en)Adyuvante completo de Freund, histopatología, HPTLC, interleucina, quercetina (es)
Adjuvante completo de Freund, histopatologia, HPTLC, interleucina, quercetina (pt)
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Introduction: rheumatoid arthritis characterized by joint inflammation and pain,
affecting millions of peoples around the world. Traditional system of medicine had
proven to be preventive and treating physical and mental illness. Objective: the
objective of the study is to assess anti-arthritic potential of the plant Rosa alba L by
considering CFA, formaldehyde and turpentine oil induced arthritic model. Material
and Method: ethanolic extract and its ethyl acetate fraction were considered for the
study and quercetin was quantitatively estimated by high performance thin layer chromatographic
(HPTLC) method. Moreover, hematological and biochemical studies
(IL-6) were performed in blood and serum of wistar albino rat. Then histopathological
studies had also been performed on rat hind paw joint. Result: phytochemical
screening estimated the presence of carbohydrates, phenolic compounds, flavonoids,
phytosterols, amino acids and fixed oil in ethanolic extract and ethyl acetate fraction of
the plant. Amount of quercetin in the flowers of the plant was found to be 0.26% w/w
by quantitative HPTLC method. The level of proinflammatory cytokines, interleukin
(IL-6) was considerably decreased (p<0.001) in the fraction treated group of rats at
400 mg/kg b.w by considering complete freund’s adjuvant (CFA) induced arthritic model.
Conclusion: Ameliorated hematological,hematological, biochemical and histopathological
parameters confirmed the antiarthritic potential of the plant.
Introducción: artritis reumatoide caracterizada por inflamación y dolor articular,
que afecta a millones de personas en todo el mundo. El sistema tradicional de medicina
había demostrado ser preventivo y tratar enfermedades físicas y mentales. Objetivo:
el objetivo del estudio es evaluar el potencial antiartrítico de la planta Rosa
alba L considerando el modelo artrítico inducido por CFA, formaldehído y aceite de
trementina. Material y método: el extracto etanólico y su fracción de acetato de etilo
se consideraron para el estudio y la quercetina se estimó cuantitativamente mediante
el método cromatográfico en capa fina de alto rendimiento (HPTLC). Además, se
realizaron estudios hematológicos y bioquímicos (IL-6) en sangre y suero de rata
albina wistar. A continuación, también se realizaron estudios histopatológicos en
la articulación de la pata trasera de rata. Resultado: el cribado fitoquímico estimó
la presencia de carbohidratos, compuestos fenólicos, flavonoides, fitoesteroles,
aminoácidos y aceite fijo en el extracto etanólico y la fracción de acetato de etilo de
la planta. Se encontró que la cantidad de quercetina en las flores de la planta era del
0,26% p / p por el método de HPTLC cuantitativo. El nivel de citocinas proinflamatorias,
interleucina (IL-6), disminuyó considerablemente (p<0,001) en el grupo
de ratas tratado con fracción a 400 mg/kg de peso corporal considerando el modelo
artrítico inducido por adyuvante de Freund (CFA) completo. Conclusión: la mejora
de los parámetros hematológicos, bioquímicos e histopatológicos confirmó el potencial
antiartrítico de la planta.
Introdução: artrite reumatóide caracterizada por inflamação e dor nas articulações,
afetando milhões de pessoas em todo o mundo. O sistema tradicional de medicina
provou ser preventivo e tratamento de doenças físicas e mentais. Objetivo: avaliar o
potencial antiartrítico da planta Rosa alba L, considerando o modelo artrítico induzido
por CFA, formaldeído e óleo de terebintina. Material e Método: o extrato
etanólico e sua fração acetato de etila foram considerados para o estudo e a quercetina
foi estimada quantitativamente pelo método de cromatografia em camada
delgada de alta eficiência (HPTLC). Além disso, estudos hematológicos e bioquímicos
(IL-6) foram realizados em sangue e soro de ratos wistar albinos. Em seguida,
estudos histopatológicos também foram realizados na articulação da pata traseira
do rato. Resultado: a triagem fitoquímica estimou a presença de carboidratos,
compostos fenólicos, flavonoides, fitoesteróis, aminoácidos e óleo fixo no extrato
etanólico e na fração acetato de etila da planta. A quantidade de quercetina nas
flores da planta foi de 0,26% p/p pelo método quantitativo de HPTLC. O nível
de citocinas pró-inflamatórias, interleucina (IL-6) foi consideravelmente diminuído
(p<0,001) no grupo de ratos tratados com fração a 400 mg/kg b.w, considerando o
modelo artrítico induzido por adjuvante completo de Freund (CFA). Conclusão: a
melhora dos parâmetros hematológicos, bioquímicos e histopatológicos confirmou
o potencial antiartrítico da planta.
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