Publicado

2016-05-01

Efecto del aceite esencial de Rosmarinus officinalis sobre Porphyromonas gingivalis cultivada in vitro

Effect of essential oil of Rosmarinus officinalis on Porphyromonas gingivalis cultivated in vitro

DOI:

https://doi.org/10.15446/rcciquifa.v45n2.59942

Palabras clave:

Rosmarinus officinalis, aceite esencial, Porphyromonas gingivalis (es)
Rosmarinus officinalis, essential oil, Porphyromonas gingivalis (en)

Descargas

Autores/as

  • Diego M. Bonilla Universidad Nacional de Colombia
  • Yulitza Mendoza Universidad Nacional de Colombia
  • Campo E. Moncada Universidad Nacional de Colombia
  • Ozkarina Murcia Universidad Nacional de Colombia
  • Ángela P. Murcia Universidad Nacional de Colombia
  • Jairo Calle Universidad Nacional de Colombia
  • Roberto Pinzón Universidad Nacional de Colombia
  • Lina Neiro Universidad Nacional de Colombia

El interés por los productos naturales como fuente de agentes antimicrobianos ha
ido en aumento. Estudios previos han demostrado que extractos de plantas pueden
inhibir la proliferación bacteriana en la cavidad oral. El objetivo de este estudio fue
determinar la susceptibilidad in vitro de Porphyromonas gingivalis ATCC 33277
frente al aceite esencial de Rosmarinus officinalis. Para la evaluación de la actividad
antimicrobiana del aceite esencial se utilizó el método cuantitativo de dilución en
agar, descrito por Clinical and Laboratory Standards Institute (CLSI) para bacterias
anaerobias. El aceite esencial de R. officinalis demostró una concentración mínima
inhibitoria (CMI) contra Porphyromonas gingivalis ATCC 33277 de 1000 μg/ml.
Estos resultados sugieren que el aceite esencial podría ser útil como un agente antibacteriano en preparaciones de uso oral.

The interest in natural products as a source of antimicrobial agents has been increasing. Previous studies have shown that plant extract may inhibit bacterial growth in the oral cavity. The aim of this study was to determine the in vitro susceptibility of Porphyromonas gingivalis ATCC 33277 against the essential oil of Rosmarinus officinalis. To evaluate the antimicrobial activity of the essential oil, quantitative agar dilution method described by the Clinical and Laboratory Standards Institute (CLSI) for anaerobic bacteria was used. The essential oil of R. officinalis showed a minimum inhibitory concentration (MIC) of 1000 µg/ml against Porphyromonas gingivalis ATCC 33277. These results suggest that essential oil of R. officinalis may be useful as an antibacterial agent in oral preparations

Descargas

Los datos de descargas todavía no están disponibles.

Citas

(1) E. Koshi, S. Rajesh, P. Koshi, P. Arunima, Risk assessment for periodontal disease, Journal of Indian Society of Periodontology, 16(3), 324 (2012).

(2) B. Paster, S. Boches, J. Galvin, R. Ericson, C. Lau, V. Levanos, A. Sahasrabudhe, F. Dewhirst, Bacterial diversity in human subgingival plaque, Journal of bacteriology, 183(12), 3770 (2001).

(3) J. Kim, S. Amar, Periodontal disease and systemic condition: A bidirectional relationship, Odontology, 94, 11 (2006).

(4) A. Buonavoglia, F. Latronico, C. Pirani, M. Greco, M. Corrente, C. Prati, Symptomatic and asymptomatic apical periodontitis associated with red complex bacteria: clinical and microbiological evaluation, Odontology, 101, 84 (2013).

(5) T.R. Mikuls, J.B. Payne, F. Yu, G.M. Thiele, R.J. Reynolds, G.W. Cannon et al., Periodontitis and Porphyromonas gingivalis in patients with rheumatoid arthritis, Arthritis & Rheumatology,66(5),1090 (2014).

(6) J. Koziel, P. Mydel, J. Potempa, The link between periodontal disease and rheumatoid arthritis:an updated review, Current Rheumatology Reports, 16(3), 5 (2014).

(7) T. Kurita-Ochiai, M. Yamamoto, Periodontal pathogens and atherosclerosis: implications of inflammation and oxidative modification of LDL, BioMed Research International, 2014, 4 (2014).

(8) A. Prakasam, S. Elavarasu, R. Natarajan, Antibiotics in the management of aggressive periodontitis, Journal of Pharmacy & Bioallied Sciences, Supplement, S253- S254, (2012).

(9) K. Shinada, M. Ueno, Y. Kawaguchi, C. Konishi, S. Takehara, S. Yokoyama, T. Zaitsu, M. Ohnuki, F. Clive, Effect of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: A randomized placebo-controlled 7-day trial, Trials, 11, 15 (2010).

(10) E. Palombo, Traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases, Evidence Based Complementary and Alternative Medicine, 2011, 2 (2011).

(11) Y. Jiang, N. Wu, Y. Jie Fu, W. Wang, M. Luo, C. Zhao, Y. Gang Zu, X. Liu, Chemical composition and antimicrobial activity of the essential oil of Rosemary, Enviromental toxicology and pharmacology, 32(1), 63 (2011).

(12) W. Bernardes, R. Lucarini, M. Tozatti, M. Souza, M. Andrade, A. da Silva, C. Gomes, A. Miller, P. Pauletti, M. Groppo, W. Cunha, Antimicrobial Activity of Rosmarinus officinalis against oral pathogens: Relevance of carnosic acid and carnosol, Chemistry & Biodiversity, 7, 1838 (2010).

(13) C. Ardila, M. Granada, I. Guzmán, Antibiotic resistance of subgingival species in chronic periodontitis patients, Journal of Periodontal Research, 45, 559 (2010).

(14) R. Jacinto, B. Gomes, C. Ferraz, A. Zaia, F. Souza, Microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and

The antimicrobial susceptibility of some isolated anaerobic, bacteria, Oral Microbiology Immunology, 18, 290 (2003).

(15) H. Wagner, “Drug Analysis Thin Layer Chromatography Atlas”, Springer-Verlag,

Berlín, 1984, p. 22-23, 30-31.

(16) Clinical and Laboratory Standards Institute, “Methods for antimicrobial susceptibility testing

Of anaerobic bacteria”, approved standard-8 ed., CLSI document M11-A8, Clinical and Laboratory Standards Institute, Wayne, PA, 2012.

(17) A. Angioni, A. Barra, E. Cereti, D. Barile, J.D. Coïsson, M. Arlorio, S. Dessi, V. Coroneo, P. Cabras, Chemical composition, plant genetic differences, antimicrobial and antifungal activity investigation of the essential oil of Rosmarinus officinalis L., Journal of Agricultural and Food Chemistry, 52, 3531 (2004).

(18) J. Sánchez, “Estudio de hojas de Chelonanthus alatus (Gentianaceae)”, tesis de grado, Universidad Nacional de Colombia, 2011, pp. 11.

(19) M. Mastrogiovanni, “Extracción, purificación y caracterización de saponinas de Quillaja brasiliensis”, tesis de grado, Universidad de la República, Uruguay, 2012, pp. 17.

(20) W. Wang, N. Wu, Y.G. Zu, Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components, Food Chemistry, 108(3), 10191022 (2008).

(21) S. Santoyo, J. Cavero, L. Jaime, E. Ibañez, F.J. Señoráns, G. Reglero, Chemical composition And antimicrobial activity of Rosmarinus Officinalis L. Essential oil obtained via supercritical fluid extraction, Journal of Food Protection, 4, 790-795 (2005).

(22) Micromedex® Solutions. “Metronidazole”, Greenwood Village, Colo: Thomson Micromedex, URL: http://www.micromedexsolutions.com ,

Consultado en junio de 2015.

(23) A.I. Hussain, F. Anwar, S.A. Shahid, A. Jabbar, S. Mahboob, P.S. Nigam, Rosmarinus Officinalis essential oil: Antiproliferative, antioxidant and antibacterial activities, Brazilian Journal of Microbiology, 41, 1076 (2010).

(24) J. Smullen, M. Finney, G.M. Storey, H.A. Foster, Prevention of artificial dental plaque formation in vitro by plant extracts, Journal of Applied Microbiology, 113, 970- 971 (2012).

(25) B. Bozin, N. Mimica-Dukic, I. Samojlik, E. Jovin, Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils, Journal of Agricultural and Food Chemistry, 55(19), 7879 (2007).

(26) A. Ait-Ouazzou, S. Lorán, M. Bakkali, A. Laglaoui, C. Rota, A. Herrera, R. Pagán, P. Conchello, Chemical composition and antimicrobial activity of essential oils

of Thymus algeriensis, Eucalyptus Globulus and Rosmarinus Officinalis from Morrocco, Journal of the Science of Food and Agriculture, 91, 2646-2648 (2011).

(27) K. Takarada, R, Kimizuka, N. Takahashi, K. Honma, K. Okuda, T. Kato, A comparison of the antibacterial efficacies of essential oils against oral pathogens, Oral Microbiology and Immunology 19, 61 (2004).

(28) A. R. Bilia, F. Santomauro, C. Sacco, M. C. Sesto, R. Donato, Essential oil or Artemisia annua L.: An extraordinary component with numerous antimicrobial properties, Evidence-Based Complementary and Alternative Medicine,159819 (2014).

Licencia

Derechos de autor 2016 Revista Colombiana de Ciencias Químico-Farmacéuticas

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.

El Departamento de Farmacia de la Facultad de Ciencias de la Universidad Nacional de Colombia  autoriza la fotocopia de artículos y textos para fines de uso académico o interno de las instituciones citando la fuente. Las ideas emitidas por los autores son responsabilidad expresa de estos y no de la revista.

Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons de Atribución 4.0 aprobada en Colombia. Consulte la normativa en: http://co.creativecommons.org/?page_id=13