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Development of a surfactant mediated method for direct monitoring of atracurium in exhaled breath condensate
Desarrollo de un método mediado por surfactante para la monitorización directa de atracurio en el condensado del aliento exhalado
Desenvolvimento de um método mediado por surfactante para monitoramento direto de atracúrio no condensado do ar exalado
Palavras-chave:
Atracurium, Tabriz University of Medical Sciences, Fluorescence enhancement, Exhaled breath condensate (en)Atracurio, Dodecil sulfato de sodio, Mejora de la fluorescencia, condensado del aliento exhalado (es)
Atracúrio, Dodecilsulfato de sódio, Aumento de fluorescência, condensação da respiração exalada (pt)
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Aim: This study aimed to establish an enhanced fluorometric assay for directly monitoring atracurium in exhaled breath condensate (EBC). The principal of the technique is the rigidity induced medium in the presence of the sodium dodecyl sulfate (SDS) surfactant which eliminates quenching results from collisional and vibrational conversions. This process causes an improvement in the emission intensity of atracurium. Methods: Fluorescence recordings were made on a spectrofluorometer at 15 ˚C at 310 nm. The crucial factors for reaching to maximum response were investigated and optimized. Results: The technique was validated and the calibration curve was linear in the range of 0.01-2.0 μg·mL-1 of atracurium with a limit of detection of 0.007 μg·mL-1. Finally, the proposed technique was used for the atracurium analysis in EBC of patients receiving the drug. Conclusion: The validated technique was found to be accurate and reliable for the quantification of atracurium from the repeatability and reproducibility points of view.
Objetivo: Este estudio tuvo como objetivo establecer un ensayo fluorométrico mejorado para monitorear directamente el atracurio en el condensado del aliento exhalado (EBC). El principio de la técnica es el medio inducido por rigidez en presencia del tensioactivo dodecilsulfato de sodio (SDS), que elimina los resultados de enfriamiento de las conversiones por colisión y vibración. Este proceso provoca una mejora en la intensidad de emisión del atracurio. Métodos: Los registros de fluorescencia se realizaron en un espectrofluorómetro a 15 °C a 310 nm. Se investigaron y optimizaron los factores cruciales para alcanzar la máxima respuesta. Resultados: La técnica fue validada y la curva de calibración fue lineal en el rango de 0,01-2,0 μg·mL-1 de atracurio con un límite de detección de 0,007 μg·mL-1. Finalmente, la técnica propuesta se utilizó para el análisis de atracurio en EBC de pacientes que recibieron el fármaco. Conclusión: La técnica validada resultó ser precisa y confiable para la cuantificación de atracurio desde los puntos de vista de repetibilidad y reproducibilidad.
Objetivo: Este estudo teve como objetivo estabelecer um ensaio fluorométrico aprimorado para monitorar diretamente o atracúrio no condensado do ar exalado (EBC). O principal da técnica é o meio induzido por rigidez na presença do surfactante dodecil sulfato de sódio (SDS) que elimina os resultados de têmpera de conversões colisionais e vibracionais. Este processo provoca uma melhoria na intensidade de emissão de atracúrio. Métodos: Os registros de fluorescência foram feitos em espectrofluorômetro a 15 °C a 310 nm. Os fatores cruciais para atingir a resposta máxima foram investigados e otimizados. Resultados: A técnica foi validada e a curva de calibração foi linear na faixa de 0,01-2,0 μg·mL-1 de atracúrio com limite de detecção de 0,007 μg·mL-1. Por fim, a técnica proposta foi utilizada para análise de atracúrio em EBC de pacientes que receberam o medicamento. Conclusão: A técnica validada mostrou-se precisa e confiável para a quantificação de atracúrio do ponto de vista da repetibilidade e reprodutibilidade.
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