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Preliminary design and evaluation of an RT-PCR assay for detecting Reptarenavirus in snakes (subfamily Boinae and families Pythonidae and Colubridae) from the Reserva Natural Bioparque Wakatá, Colombia
Diseño y evaluación preliminar de una prueba de RT-PCR para la detección de Reptarenavirus en serpientes (subfamilia Boinae y familias Pythonidae y Colubridae) de la Reserva Natural Bioparque Wakatá, Colombia
DOI:
https://doi.org/10.15446/rfmvz.v72n2.119247Keywords:
Reverse transcriptase polymerase chain reaction, Snakes, Reptarenavirus, Wild animals (en)Reacción en cadena de la polimerasa de transcriptasa inversa, Serpientes, Reptarenavirus, Animales silvestres (es)
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Inclusion body disease (IBD) is a viral infection of snakes caused by Reptarenavirus, characterized primarily by neurological signs, although it can also be subclinical. This study preliminarily designed and evaluated an RT-PCR assay to detect the virus in snakes from the Wakatá Biopark (Colombia), using two sets of primers and without a positive control. Although the results were negative and the assay proved unviable for routine use, the study generated valuable information as a basis for future projects addressing this problem and provided recommendations for the differential diagnosis of the disease.
La enfermedad de cuerpos de inclusión (ECI) es una afección viral de serpientes causada por Reptarenavirus, con signos clínicos principalmente neurológicos, aunque puede ser subclínica. Este estudio diseñó y evaluó, de forma preliminar, una prueba RT-PCR en un intento de detectar el virus en serpientes del Bioparque Wakatá (Colombia), utilizando dos sets de primers y sin un control positivo. Aunque los resultados fueron negativos y la prueba no mostró ser viable para uso rutinario, se generó información valiosa como base para futuros proyectos acerca de este problema y se hacen recomendaciones acerca del diagnóstico diferencial de la enfermedad.
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