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Assessment of the anti-inflammatory, antioxidant, and wound-healing effects of methanolic soybean seed extract in an excision wound model in albino rats
Evaluación de los efectos antiinflamatorios, antioxidantes y cicatrizantes del extracto metanólico de semillas de soja en un modelo de herida por escisión en rata albina
DOI:
https://doi.org/10.15446/rfmvz.v72n2.119384Keywords:
antioxidant, oxidative stress, management, ethnopharmacology, ethnoveterinary medicine (en)antioxidante, estrés oxidativo, gestión, etnofarmacología, medicina etnoveterinaria (es)
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Wound healing is a complex physiological process influenced by oxidative stress and inflammation. This study assessed the antioxidant, anti-inflammatory, and wound-healing effects of a methanolic extract of soybean seeds using a full-thickness excision wound model in male albino rats. Fourteen rats were randomly divided into two groups (n = 7). Under anesthesia, full-thickness skin wounds were aseptically created in the thoraco-abdominal region. Group A received sterile water (placebo) topically, while Group B received the soybean seed extract daily for 21 days. Wound healing was evaluated by macroscopic examination, measurement of wound contraction, and analysis of inflammatory and oxidative stress markers.
By day 7, wound contraction was significantly higher in the control group (60.42 ± 6.65%) compared to the extract-treated group (43.96 ± 11.58%) (p < 0.05). No significant difference was observed in the neutrophil-to-lymphocyte ratio between the two groups. However, biochemical analyses showed elevated levels of serum malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the treated group (MDA: 4.26 ± 0.39 µmol/mg; SOD: 1.43 ± 0.16 mg/mL) versus the control (MDA: 3.18 ± 0.51 µmol/ mg; SOD: 1.01 ± 0.13 mg/mL) (p < 0.05).
In conclusion, topical application of soybean seed methanolic extract did not enhance wound healing but improved antioxidant markers, indicating its potential role in mitigating oxidative stress rather than directly accelerating tissue repair.
La cicatrización de heridas es un proceso fisiológico complejo influenciado por el estrés oxidativo y la inflamación. Este estudio evaluó los efectos antioxidantes, antiinflamatorios y cicatrizantes de un extracto metanólico de semillas de soya utilizando un modelo de herida por escisión de espesor completo en ratas albinas machos. El Grupo A recibió agua estéril tópica, mientras que el Grupo B fue tratado diariamente con el extracto de semillas de soya durante 21 días. La cicatrización fue evaluada mediante examen macroscópico, medición de la contracción de la herida y análisis de marcadores inflamatorios y de estrés oxidativo.
Para el día 7, la contracción de la herida fue significativamente mayor en el grupo control (60.42 ± 6.65%) en comparación con el grupo tratado con el extracto (43.96 ± 11.58%) (p < 0.05). No se observaron diferencias significativas en la relación neutrófilo-linfocito entre los grupos. Sin embargo, los análisis bioquímicos mostraron niveles elevados de malondialdehído (MDA) en suero y actividad de superóxido dismutasa (SOD) en el grupo tratado (MDA: 4.26 ± 0.39 µmol/mg; SOD: 1.43 ± 0.16 mg/mL) frente al control (MDA: 3.18 ± 0.51 µmol/mg; SOD: 1.01 ± 0.13 mg/mL) (p < 0.05).
En conclusión, la aplicación tópica del extracto metanólico de semillas de soya no mejoró la cicatrización de heridas, pero sí incrementó los marcadores antioxidantes, lo que sugiere un posible papel terapéutico en la mitigación del estrés oxidativo más que en la aceleración directa de la reparación tisular.
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Copyright (c) 2025 Iskiil Oladehinde Oyenekan, Abdulhakeem Binhambali, Oluwatodimu Adewole Adekoya, Yusirat Olabisi Akinniyi, Damilola Popoola
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