Publicado
Efecto del ibuprofeno y paracetamol sobre la conducta de evasión y estabilidad de la membrana lisosomal de la lombriz Eisenia fetida
Effect of ibuprofen and paracetamol on the avoidance behavior and stability of the lysosomal membrane of the Eisenia fetida
DOI:
https://doi.org/10.15446/abc.v31n2.122028Palabras clave:
Biomarcador, Ecotoxicología, Fármacos, Toxicidad, Suelo (es)Biomarker, Drugs, Ecotoxicology, Toxicity, Soil (en)
Descargas
En este estudio se evaluó la toxicidad de los fármacos antiinflamatorios no esteroideos, ibuprofeno y paracetamol, a través del bioensayo de comportamiento de evasión y tiempo de retención de rojo neutro (TRRN) para evaluar la estabilidad de la membrana lisosomal sobre la lombriz de tierra Eisenia fetida. La CE80 para la evasión fueron 54,8 mg/kg para ibuprofeno y 223,8 mg/kg para paracetamol. El TRRN, la concentración más baja a la cual se observa efecto (CBOE) de la pérdida de la estabilidad de la membrana lisosomal fue a los 5 mg/kg de ibuprofeno y 100 mg/kg de paracetamol. Estos resultados, indican que la presencia de ambos fármacos en suelos afecta la selección del hábitat y provocan daño celular en E. fetida. La prueba de tiempo de retención de rojo neutro fue más sensible que el de conducta de evasión y confirma que es un biomarcador de alerta temprana de efectos tóxicos.
This study evaluated the toxicity of the non-steroidal anti-inflammatory drugs ibuprofen and paracetamol in the earthworm Eisenia fetida using the avoidance behavior test and the neutral red retention time (NRRT) assay. The EC80 values for avoidance behavior were 54.8 mg kg⁻¹ for ibuprofen and 223.8 mg kg⁻¹ for paracetamol. The NRRT assay showed lysosomal membrane destabilization at concentrations as low as 5 mg kg⁻¹ for ibuprofen and 100 mg kg⁻¹ for paracetamol, corresponding to the lowest observed effect concentrations (LOEC). These results demonstrate that the presence of both pharmaceuticals in soil affects habitat selection and induces cellular damage in E. fetida. Moreover, the NRRTassay exhibited greater sensitivity than the avoidance behavior test, confirming its value as an early-warning biomarker of toxic effects.
Referencias
Alcívar, M. A., Sendra, M., Silva, D. C. V. R., González-Ortegón, E., Blasco J., Moreno-Garrido, I. and Araújo, C. (2021) Could contamination avoidance be an endpoint that protects the environment? An overview on how species respond to copper, glyphosate, and silver nanoparticles. Toxics, 9(11), 301. https://doi.org/10.3390/toxics9110301
Alistair, B. A., Boxall, B. and Brooks, W. (2024). Pharmaceuticals and Personal Care Products in the Environment: What Progress Has Been Made in Addressing the Big Research Questions?, Environmental Toxicology and Chemistry, 43(3), 481-487. https://doi.org/10.1002/etc.5827
Aznar, R., Sánchez-Brunete, C., Albero, B., Rodríguez, J. A. and Tadeo, J. (2013). Occurrence and analysis of selected pharmaceutical compounds in soil from Spanish agricultural fields. Environmental Science and Pollution Research, 21, 4772-4782. https://doi-org.bibliotecadigital.uv.cl/10.1007/s11356-013-2438-7
Bartlett, M. D, Briones, M. J. I., Neilson, R., Schmidt, O., Spurgeon, D. and Creame, R. E. (2010). A critical review of current methods in earthworm ecology: From individuals to populations. European Journal of Soil Biology, 46, 67-73. https://doi.org/10.1016/j.ejsobi.2009.11.006
Boyle, P., Richardson, M. D., Savin, M. C., Karcher, D. E. and Potter, D. A. (2019). Ecology and management of earthworm casting on sports turf. Pest Management Science, 75, 2071-2078. https://doi.org/10.1002/ps.5479
Calisi, A., Lionetto, M. and Schettino, T. (2009). Pollutant-induced alterations of granulocyte morphology in the earthworm Eisenia foetida. Ecotoxicology and Environmental Safety, 72(5), 1369-1377. https://doi.org/10.1016/j.ecoenv.2009.03.010
Calisi, A., Lionetto, M. G. and Schettino, T. (2011) Biomarker response in the earthworm Lumbricus terrestris exposed to chemical pollutants. Science of The Total Environment, 409(20), 4456–4464. https://doi.org/10.1016/j.scitotenv.2011.06.058
Carmona, E., Andreu, V. and Picó, Y. (2014). Occurrence of acidic pharmaceuticals and personal care products in Turia River Basin: From waste to drinking water. Science of The Total Environment, 484, 53-63. https://doi.org/10.1016/j.scitotenv.2014.02.085
Chacón, F. J., Cayuela, M. L. and Sánchez-Monedero, M. A. (2022). Paracetamol degradation pathways in soil after biochar addition. Environmental Pollution, 307, 119546. https://doi.org/10.1016/j.envpol.2022.119546
Choudhury, M., Adhikari, M. D., Agarwal, S., Samanta, P., Sharma, A., Kundu, D. and Kumar, S. (2025). Pharmaceuticals and personal care products in soil: Sources, impacts and myco-remediation strategies. Emerging contaminants, 11(2), 100488. https://doi.org/10.1016/j.emcon.2025.100488
Dey, S., Bano, F. and Malik, A. (2019). Pharmaceuticals and personal care product (PPCP) contamination – a global discharge inventory. In Pharmaceutical and Personal Care Products: Waste Management and Treatment Technology. Editores Majeti Narasimha Vara Prasad, Meththika Vithanage and Atya Kapley. pp. 01-2. https://doi.org/10.1016/B978-0-12-816189-0.00001-9
Duque, T., Nuriyev. R., Römbke, J., Schäfer, R. B., Entling, M. H. (2023). Variation in the Chemical Sensitivity of Earthworms from Field Populations to Imidacloprid and Copper. Environmental Toxicology and Chemistry, 42(4), 939–947. https://doi.org/10.1002/etc.5589
Food and Agriculture Organization of the United Nations. (2015). AQUASTAT: Chile http://www.fao.org/nr/water/aquastat/countries_regions/CHL/printesp1.stm
González, M. and Fernández‐López, C. (2022). Behavior of the Uptake of Ibuprofen in Five Varieties of Horticultural Crops Irrigated with Regenerated Water. Bulletin of Environmental Contamination and Toxicology, 108, 253–259. https://doi.org/10.1007/s00128-021-03387-1
Harreus, D., Köhler, H. R. and Weeks, J. M. (1997). Combined Non-Invasive Cell Isolation and Neutral-Red Retention Assay for Measuring the Effects of Copper on the Lumbricid Aporrectodea rosea (Savigny). Bulletin of Environmental Contamination and Toxicology, 59, 44–49. https://doi.org/10.1007/s001289900441
Hønsi, T. G., Stubberud, H. E., Andersen, S., Stenersen, J. (2003). Lysosomal fragility in earthworm (Eisenia veneta) exposed to heavy metal contaminated soils from two abandoned pyrite ore mines in southern Norway. Water, Air, and Soil Pollution, 142, 27-37. https://doi.org/10.1023/A:1022003809634
Hund-Rinke, K., Lindemann, M. and Simon, M. (2005). Experiences with Novel Approaches in Earthworm Testing Alternatives. Journal of Soils and Sediments, 5(4), 233-239. https://doi.org/10.1065/jss2005.06.142
Iryna, L., Yurii, K., Oleg, D. and Angelina, K. (2016). Environmental role of earthworm (Lumbricidae) in formation of soil buffering capacity against copper contamination in remediated soil, steppe zone of Ukraine. InTech. http://dx.doi.org/10.5772/64722
International Organization for Standardization. (2008). (ISO-17512-1-2008). Soil quality – Avoidance test for determining the quality of soils and effects of chemicals on behaviour – Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei). International Organization for Standardization, Ginebra, Suiza.
Li, J., Ye, Q. and Gan, J. (2014). Degradation and transformation products of acetaminophen in soil. Water Research, 49, 44–52. https://doi.org/10.1016/j.watres.2013.11.008
McLain, N. K., Gomez, M. Y. and Gachomo, E. W. (2023). Acetaminophen Levels Found in Recycled Wastewater Alter Soil Microbial Community Structure and Functional Diversity. Microbial Ecology, 85, 1448–1462. https://doi.org/10.1007/s00248-022-02022-8
Egbeyemi, M. M., Lateef, S. A., Akinsete, S. J., Omobowale, M. O. and Abayomi, T. (2023). Health risk assessment for uptake and accumulation of pharmaceuticals in jute mallow (Corchorus olitorius) irrigated with treated hospital wastewater. Environmenta Monitoring Assessment, 15(195), 956. https://doi.org/10.1007/s10661-023-11565-3
Mkhinini, M., Boughattas, I., Alphonse, V., Livet, A., Bousserrhine, N. and Banni, M. (2019). Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei. Science of The Total Environment, 647, 1245-1255. https://doi.org/10.1016/j.scitotenv.2018.07.449
Moore, M. N., Lowe, D. and Kohler, A. (2004). Biological effects of contaminants: Measurement of lysosomal membrane stability. ICES Techniques in Marine Environmental Sciences, 36. https://doi.org/10.17895/ices.pub.5060
Moore, M. N., Allen, J. I. and McVeigh, A. (2006). Environmental prognostics: an integrated model supporting lysosomal stress responses as predictive biomarkers of animal health status. Marine Environmental Research, 61(3), 278–304. https://doi.org/10.1016/j.marenvres.2005.10.005
Nadais, H., Li, X., Alves, N., Couras, C., Andersen, H. R., Angelidaki, I. and Zhang Y. (2018). Bio-electro-Fenton process for the degradation of Non-Steroidal Anti-Inflammatory Drugs in wastewater. Chemical Engineering Journal, 338, 401–410. https://doi.org/10.1016/j.cej.2018.01.014
Pantea, E. D., Coatu, V., Damir, N. A., Oros, A., Lazar, L. and Rosoiu N. (2023). Lysosomal Membrane Stability of Mussel (Mytilus galloprovincialis Lamarck, 1819) as a Biomarker of Cellular Stress for Environmental Contamination. Toxics, 11(8), 649. https://doi.org/10.3390/toxics11080649
Palafox, A., Hernández, A., López J. y Cuevas M. (2012). Evaluación de la toxicidad de los suelos mediante bioensayos con lombrices. En Cuevas, M., Espinosa, G., Ilizaliturri, C., Mendoza, A. (Ed.), Métodos Ecotoxicológicos para la evaluación de suelos contaminados con hidrocarburos (pág. 60-69). México.
Parolini, M., Binelli, A., Cogni, D., Riva, C., Provini, A. (2009). An in vitro biomarker approach for the evaluation of the ecotoxicity of non-steroidal anti-inflammatory drugs (NSAIDs). Toxicology in Vitro 23, 935 – 942. https://doi.org/10.1016/j.tiv.2009.04.014
Parolini, M., Binelli, A., Cogni, D. and Provini, A. (2010). Multi-biomarker approach for the evaluation of the cyto-genotoxicity of paracetamol on the zebra mussel (Dreissena polymorpha). Chemosphere, 79(5), 489–498. https://doi.org/10.1016/j.tiv.2009.04.014
Pérez-Lucas, G. and Navarro, S. (2024). How Pharmaceutical Residues Occur, Behave, and Affect the Soil Environment. Journal Xenobiotic, 14(4), 1343-1377. https://doi.org/10.3390/jox14040076
Pino, M. R, Val, J., Mainar, A. M., Zuriaga, E., Español, C. and Langa, E. (2015). Acute toxicological effects on the earthworm Eisenia fetida of 18 common pharmaceuticals in artificial soil. Science of The Total Environment, 518-519, 225–237. https://doi.org/10.1016/j.scitotenv.2015.02.080
Pino-Otín, M. R., Muñiz, S., Val, J. and Navarro E. (2017). Effects of 18 pharmaceuticals on the physiological diversity of edaphic microorganisms. Science of The Total Environment, 595, 441-450. https://doi.org/10.1016/j.scitotenv.2017.04.002
Rede, D., Santos, L. H. M. L. M., Ramos, S., Oliva-Teles, F., Antão, C., Sousa, S. R. and Delerue-Matos, C. (2019). Individual and mixture toxicity evaluation of three pharmaceuticals to the germination and growth of Lactuca sativa seeds. Science of Total Environment, 673, 102 – 109. https://doi.org/10.1016/j.scitotenv.2019.03.432
Santos, V. S., Anjos, J. S. X., de Medeiros J. F. and Montagner, C. C. (2022). Impact of agricultural runoff and domestic sewage discharge on the spatial–temporal occurrence of emerging contaminants in an urban stream in São Paulo, Brazil. Environmental Monitoring Assessment, 194(637). https://doi.org/10.1007/s10661-022-10288-1
Sadzawka, A., Carrasco, M., Grez, R., Mora, M., Flores H. and Neaman A. (2006). Methods of soil analysis recommended for soils of Chile. Commission standards and accreditation. Chilean Society of Soil Science, Santiago, Chile, 164 pp.
Shaheen, J., Sizirici, B. and Yildiz, I. (2022). Fate, transport, and risk assessment of widely prescribed pharmaceuticals in terrestrial and aquatic systems: A review. Emerging Contaminants, 8, 216-228. https://doi.org/10.1016/j.emcon.2022.04.001
Sharma, K., Kaushik, G., Thotakura, N., Raza, K., Sharma, N. and Nimesh, S. (2020). Enhancement effects of process optimization technique while elucidating the degradation pathways of drugs present in pharmaceutical industry wastewater using Micrococcus yunnanensis. Chemosphere, 238, 124689. https://doi.org/10.1016/j.chemosphere.2019.124689
Ulloa, M., Bustos, V., Neaman, A. y Gaete H. (2018). Comportamiento de evasión y reproducción de la lombriz Eisenia foetida en suelos agrícolas impactados por actividades mineras. Revista Internacional de Contaminación Ambiental, 34(1). https://doi.org/10.20937/RICA.2018.34.01.03
Udovic, M. and Lestan, D. (2010). Eisenia fetida avoidance behavior as a tool for assessing the efficiency of remediation of Pb, Zn and Cd polluted soil. Environmental Pollution, 158(8), 2766-2772. https://doi.org/10.1016/j.envpol.2010.04.015
Viarengo, A., Lowe, D., Bolognesi, C., Fabbri, E. and Koehler, A. (2007). The use of biomarkers in biomonitoring: A 2-tier approach assessing the level of pollutant-induced stress síndrome in sentinel organisms. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(3), 281-300. https://doi.org/10.1016/j.cbpc.2007.04.011
Wang, H-F., Xu, J-S., Zong, K., Liang, Z-W., Li, R-F., Xue, J-F., Ding, J. and Zhao, L-S. (2024). Jujuboside B alleviates acetaminophen-induced hepatotoxicity in mice by regulating Nrf2-STING signaling pathway. Ecotoxicology and Environmental Safety, 269, 115810. https://doi.org/10.1016/j.ecoenv.2023.115810
Wang, Z., Li, Ch., Shao, Y., Xue, W., Wang, N., Xu, X. and Zhang, Z. (2021). Antioxidant defense system responses, lysosomal membrane stability and DNA damage in earthworms (Eisenia fetida) exposed to perfluorooctanoic acid: an integrated biomarker approach to evaluating toxicity. RSC Advances. 11(43), 26481-26492. https://doi.org/10.1039/D1RA04097A
Weeks, J. M. and Svendsen, C. (1996). Neutral Red Retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: a simple biomarker of exposure to soil copper. Environmental Toxicology and Chemistry, 15, 1801–1805.
Wen, H., Li, Y., Xiang, L., Zhao, H., Chen, L., Cai, Q., Li, H., Mo, C., Zhou, D., Wong, M. (2017). Toxicological effects of microcystin-LR on earthworm (Eisenia fetida) in soil. Biology and Fertility of Soils 53, 849 – 860. https://doi.org/10.1007/s00374-017-1225-x
Yang, Y., Zhang, X., Jiang, J., Han, J., Li, W., Li, X., Yee, K., Snyder, S. and Alvarez, P. J. J. (2021). Which Micropollutants in Water Environments Deserve More Attention Globally?. Environmental Science & Technology, 56(1), 13−29. https://doi.org/10.1021/acs.est.1c04250
Dimensions
PlumX
Visitas a la página del resumen del artículo
Descargas
Cómo citar
Licencia
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
1. La aceptación de manuscritos por parte de la revista implicará, además de su edición electrónica de acceso abierto bajo licencia Attribution-NonCommercial-ShareAlike 4.0 (CC BY NC SA), la inclusión y difusión del texto completo a través del repositorio institucional de la Universidad Nacional de Colombia y en todas aquellas bases de datos especializadas que el editor considere adecuadas para su indización con miras a incrementar la visibilidad de la revista.
2. Acta Biológica Colombiana permite a los autores archivar, descargar y compartir, la versión final publicada, así como las versiones pre-print y post-print incluyendo un encabezado con la referencia bibliográfica del articulo publicado.
3. Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
4. Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos institucionales, en su página web o en redes sociales cientificas como Academia, Researchgate; Mendelay) lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
