Published

2020-01-01

A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores

Estudio comparativo de las técnicas de extracción para la máxima recuperación de los compuestos bioactivos de las esporas de Ganoderma lucidum

Um estudo comparativo das técnicas de extracção para a máxima recuperação de compostos bioactivos a partir de esporos de Ganoderma lucidum

DOI:

https://doi.org/10.15446/rcciquifa.v49n1.84456

Keywords:

Antioxidants, biological activity, spore-breaking techniques, chemical analysis, Fourier-transform infrared spectroscopy, Ganodermataceae (en)
Antioxidantes, actividad biológica, técnicas de rompimiento, análisis químico, espectroscopia infrarroja por transformada de Fourier, Ganodermataceae (es)
Antioxidantes, atividade biológica, técnicas de quebra, análise química, espectroscopia de infravermelho da transformada de Fourier, Ganodermataceae (pt)

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Authors

  • Diana Carolina Saavedra Plaza Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Carlos Ricardo Soccol Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Miguel Daniel Noseda Department of Biochemistry, Federal University of Paraná-UFPR, Zip Code 81531-981, Curitiba, Paraná
  • Valcineide Oliveira de Andrade Tanobe Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Oranys Marin Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Susan Grace Karp Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Gilberto Vinicius de Melo Pereira Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Júlio Cesar de Carvalho Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR, Zip Code 81531-980, Curitiba, Paraná
  • Vanete Thomaz Soccol Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná -UFPR https://orcid.org/0000-0001-9301-541X

This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.

El objetivo de este estudio fue evaluar varios métodos de ruptura de las esporas de Ganoderma lucidum y extraer sus propiedades bioactivas. Para este propósito se evaluaron diferentes técnicas de rompimiento como: la maceración manual (RM), la maceración con esferas de diversos materiales (BR) y la exposición a microondas junto la maceración de las esporas con esferas de acero/cromo (MBR1). La ruptura de las esporas fue evaluada por espectroscopia UV-Vis, la cual mostró que las vibraciones 2955, 1642, 1240, 1080 y 1746 cm-1 correspondieron a cambios estructurales en las paredes de las esporas. El extracto MBR1 presento el mayor contenido de carbohidratos (19,80 mg.g-1) y polifenoles (2,21 mg.g-1), mientras que el extracto BR tuvo una mayor actividad antioxidante (57,22% Inb DPPH). Los extractos MBR1 y BR también presentaron en el análisis de monosacáridos un 62,2 y 73,5% de contenido glucosa. Como conclusión la mejor metodología para extraer biomoléculas de las paredes de las esporas de G. lucidum fueron el tratamiento térmico con microondas y la ruptura de las paredes con esferas de acero/cromo, porque este proceso permitió la extracción de una mayor cantidad de carbohidratos con posibles propiedades antioxidantes.

O objetivo deste estudo foi avaliar vários métodos para quebrar os esporos de Ganoderma lucidum e extrair suas propriedades bioativas. Para este fim foram avaliadas diferentes técnicas de quebra como: maceração manual (RM), maceração com esferas de vários materiais (BR) e exposição a microondas juntamente com maceração de esporos com esferas de aço/crómio (MBR1). A ruptura dos esporos foi avaliada por espectroscopia UV-Vis, que mostrou que as vibrações 2955, 1642, 1240, 1080 e 1746 cm-1 correspondiam a mudanças estruturais nas paredes dos esporos. O extrato MBR1 tinha o maior teor de carboidratos (19,80 mg.g-1) e polifenóis (2,21 mg.g-1), enquanto o extrato BR tinha a maior atividade antioxidante (57,22% Inb DPPH). Os extratos MBR1 e BR também mostraram 62,2 e 73,5% de glicose na análise de monossacarídeos. Como conclusão, a melhor metodologia para extrair biomoléculas das paredes de esporos de G. lucidum foi o tratamento térmico com microondas e a quebra das paredes com esferas de aço/cromo, pois este processo permitiu a extração de uma maior quantidade de carboidratos com possíveis propriedades antioxidantes.


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How to Cite

APA

Saavedra Plaza, D. C., Soccol, C. R., Noseda, M. D., Oliveira de Andrade Tanobe, V., Marin, O., Karp, S. G., de Melo Pereira, G. V., de Carvalho, J. C. and Thomaz Soccol, V. (2020). A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores. Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(1). https://doi.org/10.15446/rcciquifa.v49n1.84456

ACM

[1]
Saavedra Plaza, D.C., Soccol, C.R., Noseda, M.D., Oliveira de Andrade Tanobe, V., Marin, O., Karp, S.G., de Melo Pereira, G.V., de Carvalho, J.C. and Thomaz Soccol, V. 2020. A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores. Revista Colombiana de Ciencias Químico-Farmacéuticas. 49, 1 (Jan. 2020). DOI:https://doi.org/10.15446/rcciquifa.v49n1.84456.

ACS

(1)
Saavedra Plaza, D. C.; Soccol, C. R.; Noseda, M. D.; Oliveira de Andrade Tanobe, V.; Marin, O.; Karp, S. G.; de Melo Pereira, G. V.; de Carvalho, J. C.; Thomaz Soccol, V. A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores. Rev. Colomb. Cienc. Quím. Farm. 2020, 49.

ABNT

SAAVEDRA PLAZA, D. C.; SOCCOL, C. R.; NOSEDA, M. D.; OLIVEIRA DE ANDRADE TANOBE, V.; MARIN, O.; KARP, S. G.; DE MELO PEREIRA, G. V.; DE CARVALHO, J. C.; THOMAZ SOCCOL, V. A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 49, n. 1, 2020. DOI: 10.15446/rcciquifa.v49n1.84456. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/84456. Acesso em: 8 mar. 2025.

Chicago

Saavedra Plaza, Diana Carolina, Carlos Ricardo Soccol, Miguel Daniel Noseda, Valcineide Oliveira de Andrade Tanobe, Oranys Marin, Susan Grace Karp, Gilberto Vinicius de Melo Pereira, Júlio Cesar de Carvalho, and Vanete Thomaz Soccol. 2020. “A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores”. Revista Colombiana De Ciencias Químico-Farmacéuticas 49 (1). https://doi.org/10.15446/rcciquifa.v49n1.84456.

Harvard

Saavedra Plaza, D. C., Soccol, C. R., Noseda, M. D., Oliveira de Andrade Tanobe, V., Marin, O., Karp, S. G., de Melo Pereira, G. V., de Carvalho, J. C. and Thomaz Soccol, V. (2020) “A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores”, Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(1). doi: 10.15446/rcciquifa.v49n1.84456.

IEEE

[1]
D. C. Saavedra Plaza, “A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores”, Rev. Colomb. Cienc. Quím. Farm., vol. 49, no. 1, Jan. 2020.

MLA

Saavedra Plaza, D. C., C. R. Soccol, M. D. Noseda, V. Oliveira de Andrade Tanobe, O. Marin, S. G. Karp, G. V. de Melo Pereira, J. C. de Carvalho, and V. Thomaz Soccol. “A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores”. Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 49, no. 1, Jan. 2020, doi:10.15446/rcciquifa.v49n1.84456.

Turabian

Saavedra Plaza, Diana Carolina, Carlos Ricardo Soccol, Miguel Daniel Noseda, Valcineide Oliveira de Andrade Tanobe, Oranys Marin, Susan Grace Karp, Gilberto Vinicius de Melo Pereira, Júlio Cesar de Carvalho, and Vanete Thomaz Soccol. “A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores”. Revista Colombiana de Ciencias Químico-Farmacéuticas 49, no. 1 (January 1, 2020). Accessed March 8, 2025. https://revistas.unal.edu.co/index.php/rccquifa/article/view/84456.

Vancouver

1.
Saavedra Plaza DC, Soccol CR, Noseda MD, Oliveira de Andrade Tanobe V, Marin O, Karp SG, de Melo Pereira GV, de Carvalho JC, Thomaz Soccol V. A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 2020 Jan. 1 [cited 2025 Mar. 8];49(1). Available from: https://revistas.unal.edu.co/index.php/rccquifa/article/view/84456

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1. Nguyen Huu Lac Thuy, Vo Linh Tu, Le Nguyen Anh Thu, Tran Thanh Giang, Dao Tang Khanh Huyen, Duong Hoang Loc, Dao Ngoc Hien Tam, Nguyen Tuan Phat, Hong-Han Huynh, Thien Tan Tri Tai Truyen, Quang-Hien Nguyen, Uyen Do, Dang Nguyen, Truong Van Dat, Le Huu Nhat Minh. (2023). Pharmacological Activities and Safety of Ganoderma lucidum Spores: A Systematic Review. Cureus, https://doi.org/10.7759/cureus.44574.

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