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The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser
Efecto de la concentración de material inerte en un biocatalizador de alginato de calcio con células inmovilizadas sobre la fermentación láctica
DOI:
https://doi.org/10.15446/ing.investig.v25n2.14644Keywords:
lactic acid, fermentation, biocatalyst, immobilised cells, calcium alginate (en)lactic acid, fermentation, biocatalyst, immobilised cells, calcium alginate (es)
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Colombia is one of the world’s main sugarcane cultivating countries but it has not diversified its fermentation industry; a few fermentation industries produce alcohol and yeasts. Lactic acid and its derivatives then become alternatives providing added value to the sugar produced, thus benefiting the regions producing the sugar. This work evaluated the kinetics of lactic acid production using immobilized cells in calcium alginate at different concentrations of inert material. Lactobacillus delbrueckii was the microorganism used and fermentation broth mainly consisted of sucrose and yeast exact. CSTR reactors were used without pH control. The results suggested that 2% to 3% inert material in the biocatalyst increased cellular retention and diffusiveness, leading to improved conversion and reaction rate.
Colombia es uno de los principales cultivadores mundiales de caña de azúcar y no ha desarrollado una industria de fermentación diversificada, centrándose principalmente en la producción de alcoholes y levaduras. El ácido láctico y sus derivados, constituyen una alternativa que da mayor valor agregado al azúcar producido y con ello se beneficiaria a las regiones que lo producen. En el presente trabajo se evaluó la cinética de producción de ácido láctico utilizando células inmovilizadas en alginato de calcio con un inerte con características zeolíticas en diterentes concentraciones. Se empleó como microorganismo Lactobacillus delbrueckii en un medio compuesto principalmente por sacarosa y extracto de levadura. Para los ensayos se emplearon reactores de mezcla total sin control de pH. Los resultados indican que un porcentaje de inerte entre 2 y 3% mejora la retención celular y la difusividad, produciendo por consiguiente mayores conversiones y velocidades de reacción.
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Copyright (c) 2005 Juan Carlos Serrato, Luis A Caicedo Mesa
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