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2012-01-01
PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO
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Producing polyclonal antibodies (IgY) in
chickens has advantages over those obtained
in other animal models, since they
have been used as a tool for studying
different proteins (NMDA glutamate receptor
in our case, specifically the NR1
subunit). We produced specific antibodies
against expression products by the
alternative splicing of the gene encoding
NMDA receptor NR1 subunit in adult rat
brain. Three peptides corresponding to
the splicing sites (N1, C1 and C2’ cassettes)
were designed, synthesised and used
individually as antigens in hens. Specific
immunoglobulins were purified from
yolks. The antibodies were then used for
purifying the NMDA receptor NR1 subunit
using affinity chromatography coupling
the three antibodies to the support.R
How to Cite
IEEE
[1]
E. A. Reyes Montaño, L. Lareo, and G. Pérez, “PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO”, Rev. Colomb. Quim., vol. 41, no. 1, pp. 31–45, Jan. 2012.
ACM
[1]
Reyes Montaño, E.A., Lareo, L. and Pérez, G. 2012. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO. Revista Colombiana de Química. 41, 1 (Jan. 2012), 31–45.
ACS
(1)
Reyes Montaño, E. A.; Lareo, L.; Pérez, G. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO. Rev. Colomb. Quim. 2012, 41, 31-45.
APA
Reyes Montaño, E. A., Lareo, L. and Pérez, G. (2012). PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO. Revista Colombiana de Química, 41(1), 31–45. https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306
ABNT
REYES MONTAÑO, E. A.; LAREO, L.; PÉREZ, G. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO. Revista Colombiana de Química, [S. l.], v. 41, n. 1, p. 31–45, 2012. Disponível em: https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306. Acesso em: 11 aug. 2024.
Chicago
Reyes Montaño, Edgar Antonio, Leonardo Lareo, and Gerardo Pérez. 2012. “PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO”. Revista Colombiana De Química 41 (1):31-45. https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306.
Harvard
Reyes Montaño, E. A., Lareo, L. and Pérez, G. (2012) “PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO”, Revista Colombiana de Química, 41(1), pp. 31–45. Available at: https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306 (Accessed: 11 August 2024).
MLA
Reyes Montaño, E. A., L. Lareo, and G. Pérez. “PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO”. Revista Colombiana de Química, vol. 41, no. 1, Jan. 2012, pp. 31-45, https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306.
Turabian
Reyes Montaño, Edgar Antonio, Leonardo Lareo, and Gerardo Pérez. “PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO”. Revista Colombiana de Química 41, no. 1 (January 1, 2012): 31–45. Accessed August 11, 2024. https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306.
Vancouver
1.
Reyes Montaño EA, Lareo L, Pérez G. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO. Rev. Colomb. Quim. [Internet]. 2012 Jan. 1 [cited 2024 Aug. 11];41(1):31-45. Available from: https://revistas.unal.edu.co/index.php/rcolquim/article/view/30306
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