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Detección del antígeno Tn en tumores epiteliales con la lectina de Vicia villosa isolectina B4.
Using Vicia villosa lectin (B4 isolectin) for detecting Tn antigen in epithelial tumours
Palabras clave:
antígenos, lectinas, células epiteliales, cuello utero, neoplasias del cuello uterino. (es)antigens, lectins, epithelial cells, cervix uteri, uterine cervical neoplasms. (en)
Antecedentes Los epítopes T, Tn y sTn, se expresan en un alto porcentaje de tumores epiteliales y pueden detectarse con anticuerpos monoclonales y lectinas. Objetivo. Evaluar diferencias de expresión del antígeno Tn en cortes histológicos de epitelios no neoplásicos y tumores epiteliales mediante isolectina B4 de Vicia villosa.
Material y métodos. Se evaluaron semicuantitativamente localización, intensidad y porcentaje de expresión del antígeno en carcinomas in-situ e infiltrantes y epitelios no neoplásicos de cérvix, seno y urotelio, mediante isolectina B4.
Resultados La expresión de Tn en cérvix predominó en membrana de células no neoplásicas y citoplasma de células tumorales; su intensidad fue mayor en carcinomas in-situ e infiltrantes comparado con epitelio no neoplásico aunque en este el porcentaje de expresión fue mayor. En seno, la expresión de Tn fue predominantemente citoplasmática con intensidad similar, el porcentaje de expresión fué mayor en carcinomas ductales in-situ e infiltrantes. En urotelio no neoplásico y tumoral la expresión de Tn predominó en citoplasma; la intensidad y el porcentaje de expresión fueron mayores en neoplasias no invasivas de bajo y alto grado, mientras que en urotelio no neoplásico fue baja y no hubo tendencia definida en tumores infiltrantes.
Conclusiones. La detección del antígeno Tn mediante la lectina VVB4 mostró una mayor extensión de marcación en carcinomas ductales de seno en relación con el epitelio no neoplásico, pero no mostró una tendencia definida entre el tejido normal, ni diferentes etapas del desarrollo de los tumores de cérvix y urotelio. Estos hallazgos pueden atribuirse a la heterogeneidad de los procesos carcinogénicos o a que la especificidad de la lectina VVB4 no está restringida a este antígeno.
Background. T, Tn and sTn epitopes are expressed in a large percentage of epithelial tumours and may be detected with monoclonal antibodies and lectins.
Objective. Assess differences in expression of Tn antigen in histological sections of non- neoplastic epithelia and epithelial tumors by isolectin B4 from Vicia villosa
Materials and methods. The localisation, intensity and percentage of antigen expression in in-situ and infiltrant carcinomas and non-neoplasic epithelial cells from the cervix, breast and urothelium were semi-quantitatively evaluated by B4 isolectin.
Results. Tn expression in the cervix predominated in the membrane of non-neoplasic cells and the cytoplasm of tumour cells; its intensity was greater in in-situ and infiltrant carcinomas compared to non-neoplasic epithelial cells, even though such percentage of expression was greater. Tn expression in the breast was predominantly cytoplasmic (having similar intensity). The percentage of expression was greater in in-situ ductal carcinomas and infiltrates. Tn expression in non-neoplasic and tumoural urothelium predominated in the cytoplasm; the intensity and percentage of expression were greater in low and high degree non-invasive neoplasias, whilst this was low in non-neoplasic urothelium and there was no defined tendency in infiltrant tumours.
Conclusions. Tn antigen detection by lectin VVB4 showed greater expression in breast ductal carcinomas in relation to non-neoplastic epithelium, but showed on definite trend between the normal tissue and different stages of development of cervical tumors and urothelium. These findings can be related to heterogeneity of the carcinogenic processes or may be attributed to the specificity of the lectin VVB4 is not restricted to Tn antigen.
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Derechos de autor 2010 Revista de la Facultad de Medicina
Esta obra está bajo una licencia Creative Commons Reconocimiento 3.0 Unported.
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