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Sobrepeso y expresión de receptores de adiponectina en monocitos de sangre periférica
Overweight and adiponectin receptors expression in peripheral blood monocytes
Palabras clave:
adiponectina, sobrepeso, monocitos, reacción en cadena de la polimerasa de transcriptasa reversa (RT-PCR), expresión génica. (es)adiponectin, overweight, monocytes, reverse transcriptase polymerase chain reaction, gene expression, receptors. (en)
Objetivo. Determinar la expresión relativa de los receptores 1 y 2 de adiponectina (AdipoR1 y AdipoR2) en monocitos circulantes de sujetos con sobrepeso y con peso normal.
Material y métodos. Mediante reacción en cadena de la polimerasa en tiempo real se determinó la expresión relativa (mRNA) de AdipoR1 y AdipoR2 en monocitos de sangre periférica aislados con técnica de inmunoafinidad, en un grupo de estudiantes de 18 a 25 años (n=48); además se midieron parámetros antropométricos y bioquímicos (resistencia a la insulina con modelo homeostático, colesterol total, colesterol-HDL y triglicéridos).
Resultados. Se encontró que los niveles de expresión de AdipoR1 en monocitos eran mayores que los de AdipoR2 (p < 0,001). No se observaron diferencias en la expresión de AdipoR1 o AdipoR2 en monocitos de acuerdo al género, índice de masa corporal, porcentaje de masa grasa, resistencia a la insulina o perfil lipídico.
Conclusión. El hallazgo de una mayor expresión de AdipoR1 en comparación a AdipoR2 en monocitos circulantes corrobora los resultados de otros autores. Los datos obtenidos sugieren que la expresión de AdipoR1 y AdipoR2 en monocitos de sujetos normales y con sobrepeso no se relaciona con la sensibilidad a la insulina. Se recomienda realizar otros estudios, que además de involucrar población con índice de masa corporal superior a 30 kg/m2 e individuos con obesidad abdominal, determinen posibles efectos de factores relacionados con el estilo de vida sobre la regulación de la expresión de estos receptores en monocitos circulantes.
Aims. To determine AdipoR1 and AdipoR2 relative expression in peripheral blood monocytes from overweight and normal subjects.
Materials and methods. AdipoR1 and AdipoR2 relative expression was determined in peripheral blood monocytes by using real-time polymerase chain reaction. Peripheral blood monocytes were isolated by means of immunoaffinity technique from a group of students aged 18 to 25 years (n=48). Anthropometric and biochemical parameters (total and HDL-cholesterol, triglycerides and insulin resistance estimated by the homeostasis model assessment ratio) were measured.
Results. AdipoR1 expression in peripheral blood monocytes was higher than that of AdipoR2 (p < 0.001). According to sex, body mass index, percentage body fat, insulin resistance or lipid profile, there were no differences in AdipoR1 or AdipoR2 expression.
Conclusions. Our results about a greater AdipoR1 expression in comparison to AdipoR2 corroborate previous findings. Our data suggest no relation between the expression of AdipoR1 and AdipoR2 in monocytes of normal and overweighted subjects and insulin sensibility. Further studies are recommended which involve population with body mass index superior to 30 kg/m2 and individuals with abdominal obesity, and in addition establish possible effects of style-life related factors on the regulation of the expression of these receptors in peripheral monocytes.
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Derechos de autor 2007 Revista de la Facultad de Medicina
Esta obra está bajo una licencia Creative Commons Reconocimiento 3.0 Unported.
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