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Comparison of hemocytes of 5th-instar nymphs of Rhodnius prolixus (Stal) and Rhodnius robustus (Larousse 1927), before and after molting
Comparación de los hemocitos de las ninfas de V instar de Rhodnius prolixus (Stål) and Rhodnius robustus, antes y después de la muda (Larousse 1927)
Palabras clave:
Rhodnius prolixus, hemocytes, molt, Rhodnius robustus (en)Rhodnius prolixus, hemocitos, muda (es)
Background. The immune response of insects involves humoral factors and cellular elements known as hemocytes. There are different reports that explore the response of hemocytes to infections, but the effect that molting has on this response has not been explored so far. We hypothesized that there would be a change in the percentage of hemocytes as a response to the molting process.
Objective. The aim of this work was to compare the hemogram (CBC), the formula, and the differential count of hemocytes in IV instar before molting and in V instar nymphs 24 hours after molting in two species: Rhodnius prolixus and Rhodnius robustus.
Materials and methods. We assayed different staining methodologies including Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, Gomori substrate, PAS (Schiff), Sudan Black and Papanicolau with positive controls for each one. In the Gomori staining, we observed lysosomes in the granulocytes and plasmatocytes, but the differentiation was better detected using Giemsa staining.
Results. There were no statistically significant differences between the two species studied in plasmatocytes (p=0,053) or even in granulocytes (p=0,5). However, differences were significant in the prohemocytes (p=0,001) during the molting process in both Rhodnius prolixus and Rhodnius robustus.
Conclusions. Significant differences in prohemocytes between nymphs of IV and V instar were detected. No significant differences in the amount of cells were observed between the two species and the two stages. These findings may be explained due to their role as precursor cell of prohemocytes.
Antecedentes. La respuesta inmune de los insectos involucra factores humorales y elementos celulares llamados hemocitos. Existen varios trabajos explorando la respuesta de los hemocitos frente a infecciones, pero no como respuesta al proceso de muda.
Objetivo. Comparar el hemograma: la fórmula y el recuento diferencial de hemocitos de Rhodnius prolixus y Rhodnius robustus en ninfas de IV estadio antes de la muda y ninfas de V estadio 24 horas después de la muda.
Materiales y métodos. Se ensayaron las coloraciones Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, sustrato Gomori, PAS (Schiff), Sudán Negro, Papanicolau, con controles positivos para cada una. Con la coloración de Gomori se observaron lisosomas en los granulocitos y en plasmatocitos, pero la diferenciación se logró mejor con la coloración de Giemsa.
Resultados. Los plasmatocitos fueron más abundantes en las ninfas de IV estadio de Rhodnius robustus y en las de V estadio de las dos especies, pero en las ninfas de V estadio de R. prolixus, el recuento de prohemocitos fue mayor; para los oenocitoides, esferulocitos y adipohemocitos el recuento no alcanzó al 1 %. En las dos especies no se encontraron diferencias estadísticamente significativas en plasmatocitos (p=0,05319), ni en granulocitos (p=0,5), entre las ninfas de IV y V estadio, pero fue significativa en prohemocitos (p=0,001).
Conclusiones. Se detectaron diferencias significativas en prohemocitos entre las ninfas de IV y V estadio. No hubo un aumento significativo en el conteo de células en los diferentes estadios.
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Williams MJ. Drosophila Hemopoiesis and Cellular Immunity. J Immunol. 2007;178:4711-6. doi: http://doi.org/zf9.
Silverstein SC. Phagocitosis of microbes: insights and prospects. Trends cell Biol. 1995;5(3):141-2. doi: http://doi.org/fmktfc.
Kurtz J. Phagocitosis by invertebrate hemocytes: causes of individual variation in Panorpia vulgaris scorpion flies. Micros Res Tech. 2002;57(6):456-68. doi: http://doi.org/dcws99.
Mandato CA, Diehl-Jones WL, Moore SJ, Downer RGH. The effects of eicosanoid biosynthesis inhibitors on prophenoloxidase activation, phagocytosis and cell spreading in Galleria melonella. J Insect Physiol. 1997;43(1):1-8. doi: http://doi.org/dt2xjk.
Holz A, Bossinger B, Strasser T, Janning W, Klapper R. The two origins of hemocytes in Drosophila. Development. 2003;130(20):4955-62. doi: http://doi.org/brfkz7.
Honti V, Csordas G, Hurucz E, Markus R, Ando I. The cell-mediated immunity of Drosophila melanogaster: hemocyte lineages, immune compartments microanatomy and regulation. Develop Comp Immunol. 2014;42(1):47-56. doi: http://doi.org/zgb.
Nardi JB, Miklasz SD. Hemocytes contribute to both the formation and breakdown of the basal lamina in developing wings of Manduca sexta. Tissue and Cell. 1989;21(4):559-67. doi: http://doi.org/cfvwkj.
Beetz S, Brinkmann M, Trenczeck T. Differences between larval and pupa hemocytes of the tobacco hornworm, Manduca sexta, determined by monoclonal antibodies and density centrifugation. J Ins Pathol. 2004;50(9):805-19. doi: http://doi.org/d7hbmt.
Jones JC. The hemocytes of Rhodnius prolixus Stål. Biol Bol. 1965;129(2):282-94. doi: http://doi.org/cf436q.
Amaral IMR, Neto JFM, Pereira JB, Franco MR, Beletti ME, Kerr WE, Ueira-Vieira C. Circulating hemocytes fron larvae of Melipona scutellaris (Hymenoptera,Apidae,Meliponini): Cell types and their role in phagocytosis. Micron. 2010; 41(2):123-9. doi: http://doi.org/d6n2jq.
De Azambuja P, Garcia ES, Ratcliffe NA. Aspects of Classification of Hemiptera Hemocytes from six Triatomine Species. Mem Inst Oswaldo Cruz. 1991; 86(1):1-10. doi: http://doi.org/czjx42.
Kwon H, Bang K, Cho S. Characterization of the hemocytes in larvae of Protaetia brevitarseis seulensis: involvement of granulocyte-mediated phagocytosis. Plos One. 2014;9(8):1-12. doi: http://doi.org/zgc.
Giulianini PG, Bertolo F, Battistella S, Amirante GA. Ultrastructure of the hemocytes of Cetonischema aeruginosa larvae (Coleoptera: Scarabidae) involvement of both granulocytes and oenocytoids in vivo phagocytosis. Tissue Cell. 2003;35(4):243-51. doi: http://doi.org/dm5rws.
Ling F, Yu XQ. Hemocytes from the tobacco hornworm Manduca sexta have distinct function in phagocytosis of foreign particles and self dead cells. Dev Comp Immunol. 2005;30(3):301-09. doi: http://doi.org/cbsprm.
Aladaileh S, Nair SV, Birch D, Raftos DA. Sydney rock oyster (Saccostrea glomerata) hemocytes: Morphology and function. J Invertebr Pathol. 2007;96(1):48-63. doi: http://doi.org/brt3j7.
Barraco MA, Loch CT. Ultrastuctural Studies of the Hemocytes of Panstrongylus megistus (Hemiptera:Reduviidae). Mem Inst Oswaldo Cruz. 1989;84:171-88. doi: http://doi.org/c7xwtp.
Stoepler TM, Castillo JC, Lili JT, Eleftherianos I. Hemocyte density increases with developmental stage in an immune-challenged forest caterpillar. PlosOne 2013;8(8):1-8. doi: http://doi.org/zgd.
Sorrentino RP, Carton Y, Govind S. Cellular Immune Response to parasite Infection in the Drosophila Lymph Gland is Developmentally Regulated. Develop Biol. 2002;243(1):65-80. doi: http://doi.org/dgk6nn.
Theopold U, Schmidt O, Söderhäll K, Dushay M. Coagulation in arthropods: defence, wound closure and healing. Trends in Immunol. 2004;25(6):289-94. doi: http://doi.org/fnxjz2.
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Derechos de autor 2015 Revista de la Facultad de Medicina
Esta obra está bajo una licencia Creative Commons Reconocimiento 3.0 Unported.
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