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Gas chromatography determination of fatty acid alkyl esters (methyl and ethyl) in the presence of mono-, di- and triglycerides
Determinación por cromatografía de gases de alquil ésteres (metílico y etílico) de ácidos grasos, en presencia de mono-, di- y triglicéridos
DOI:
https://doi.org/10.15446/ing.investig.v25n1.14625Keywords:
methanolysis, ethanolysis, gas chromatrography (en)metanólisis, etanólisis, cromatografía de gases (es)
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Determining fatty acid methyl or ethyl esters, in the presence of mono-, di- and tri glycerides, is very important when studying fatty compounds’ methanolysis or ethanolysis, as well as for controlling the quality of petrochemical products. This work presents a useful technique for determining fatty acids methyl or ethyl esters by high temperature gas chromatography in the presence of mono-, di- and triglycerides. Samples were silylated with N, O-bis (trimethylsilyl) tritluoroacetamide (BSTFA) and then passed through a 12 m HT5 column coated with a phenylpolysiloxane-carborane film. Standard methyl and ethyl palmitate, methyl and ethyl oleate, DL-α palmitin, dipalmitin, tripalmitin and triolein solutions were used for calibrating the technique, using tricaprin as internal standard. Retention times and response factors were also determined. The results were employed in following-up palm oil methanolysis and ethanolysis reactions.
La determinación de ésteres metílicos o etílicos de ácidos grasos, en presencia de mono-, di- y triglicéridos, es muy importante para el estudio de los procesos de metanólisis o etanólisis de compuestos grasos, y para el control de calidad de productos oleoquímicos. En este trabajo se presenta una técnica útil para la determinación por cromatografía de gases de alta temperatura de ésteres metílicos o etílicos de ácidos grasos, en presencia de mono-, di- y triglicéridos. Las muestras se sililaron con N, O-bis (trimetilsilil) trifluoroacetamida (BSTFA), y posteriormente se pasaron por una columna HT5 de 12 m, recubierta con una película de fenil-polisiloxanocarburano. En la calibración de la técnica se emplearon soluciones estándar de palmitato de metilo y etilo, oleato de metilo y etilo, DL- α palmitina, dipalmitina, tripalmitina y trioleina, con tricaprina como estándar interno, y se determinaron los tiempos de retención y los factores de respuesta. Los resultados se emplearon en el seguimiento de reacciones de metanólisis y etanólisis de aceite de palma.
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References
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Copyright (c) 2005 Paulo César Narváez Rincón, Jesús Alfonso Torres, Francisco José Sánchez Castellanos, Luisa Fernanda Ponce de León
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