Published
Gas chromatography determination of fatty acid alkyl esters (methyl and ethyl) in the presence of mono-, di- and triglycerides
Determinación por cromatografía de gases de alquil ésteres (metílico y etílico) de ácidos grasos, en presencia de mono-, di- y triglicéridos
DOI:
https://doi.org/10.15446/ing.investig.v25n1.14625Keywords:
methanolysis, ethanolysis, gas chromatrography (en)metanólisis, etanólisis, cromatografía de gases (es)
Downloads
Determining fatty acid methyl or ethyl esters, in the presence of mono-, di- and tri glycerides, is very important when studying fatty compounds’ methanolysis or ethanolysis, as well as for controlling the quality of petrochemical products. This work presents a useful technique for determining fatty acids methyl or ethyl esters by high temperature gas chromatography in the presence of mono-, di- and triglycerides. Samples were silylated with N, O-bis (trimethylsilyl) tritluoroacetamide (BSTFA) and then passed through a 12 m HT5 column coated with a phenylpolysiloxane-carborane film. Standard methyl and ethyl palmitate, methyl and ethyl oleate, DL-α palmitin, dipalmitin, tripalmitin and triolein solutions were used for calibrating the technique, using tricaprin as internal standard. Retention times and response factors were also determined. The results were employed in following-up palm oil methanolysis and ethanolysis reactions.
La determinación de ésteres metílicos o etílicos de ácidos grasos, en presencia de mono-, di- y triglicéridos, es muy importante para el estudio de los procesos de metanólisis o etanólisis de compuestos grasos, y para el control de calidad de productos oleoquímicos. En este trabajo se presenta una técnica útil para la determinación por cromatografía de gases de alta temperatura de ésteres metílicos o etílicos de ácidos grasos, en presencia de mono-, di- y triglicéridos. Las muestras se sililaron con N, O-bis (trimetilsilil) trifluoroacetamida (BSTFA), y posteriormente se pasaron por una columna HT5 de 12 m, recubierta con una película de fenil-polisiloxanocarburano. En la calibración de la técnica se emplearon soluciones estándar de palmitato de metilo y etilo, oleato de metilo y etilo, DL- α palmitina, dipalmitina, tripalmitina y trioleina, con tricaprina como estándar interno, y se determinaron los tiempos de retención y los factores de respuesta. Los resultados se emplearon en el seguimiento de reacciones de metanólisis y etanólisis de aceite de palma.
References
Bailey, A., Bailey’s Industrial Oil and Fat Products, Volume 5, Edited by Hui, Y. H., John Wiley & Sons, USA, 1996.
Darnoko D.; Cheryan, M. “Kinetics of palm oil transesterification in a batch reactor”. J. Am. Oil Chem. Soc., 77 (12), 1262-1266, 2000.
Filliers, R.; Benjelloun-Mlayah; M., Delmas, M., “Ethanolysis of rapeseed Oil: Quantitation of Ethyl Esters, Mono- Di- and triglycerides and Glycerol by High performance Size Exclusion Chromatography”, J. Am. Oil Chem. Soc., 72 (4), 427-432, 1995.
Freedman, B.; Pryde, E. H.; Mounts, T. L., “Variables affecting the yields of fatty esters from transesterified vegetable oils”, J. Am. Oil Chem. Soc., 61 (10), 1638-1643, 1984.
Freedman, B.; Kwolek, W.; Pryde, E., “Quantitation in Analysis of Transesterifies Soybean Oil by Capillary Gas Chromatography”, J. Am. Oil Chem. Soc., 63 (10), 1370-1375, 1986.
Freedman, B., Butterfield, R., Pryde, E. (1986). Transesterification Kinetics of Soybean, J. Am. Oil Chem. Soc., 63 (10), 1375-1380.
Gunstone, F.; Hamilton, R.; Oleochemical Manufacture and Applications, Sheffield Academic Press, Sheffield, 2001.
Heiden, R., “Analytical Methodologies for the determination of biodiesel ester purity - determination of total methyl esters”, final report, Contract, 520320-1 NBB, February 27, pp.27, 1996.
Plank, C.; Lorbeer, E., “Simultaneous determination of glycerol, and mono-, di- and triglycerides in vegetable oil methyl esters by capillary gas chromatography”, Journal of Chromatography A, 697, 461-468, 1995.
Shumacher, L. G.; Elser, N., Sample Analysis from Biodiesel Fuel, National Biodiesel Board, Columbia, Missouri, pp. 7, 1997.
Takano, S.; Kondoh, Y., “Monoglyceride Analysis with Reversed Phase HPLC”, J. Am. Oil Chem. Soc, 64 (7), 1000-1003, 1987.
Dimensions
PlumX
Article abstract page views
Downloads
How to Cite
License
Copyright (c) 2005 Paulo César Narváez Rincón, Jesús Alfonso Torres, Francisco José Sánchez Castellanos, Luisa Fernanda Ponce de León
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors or holders of the copyright for each article hereby confer exclusive, limited and free authorization on the Universidad Nacional de Colombia's journal Ingeniería e Investigación concerning the aforementioned article which, once it has been evaluated and approved, will be submitted for publication, in line with the following items:
1. The version which has been corrected according to the evaluators' suggestions will be remitted and it will be made clear whether the aforementioned article is an unedited document regarding which the rights to be authorized are held and total responsibility will be assumed by the authors for the content of the work being submitted to Ingeniería e Investigación, the Universidad Nacional de Colombia and third-parties;
2. The authorization conferred on the journal will come into force from the date on which it is included in the respective volume and issue of Ingeniería e Investigación in the Open Journal Systems and on the journal's main page (https://revistas.unal.edu.co/index.php/ingeinv), as well as in different databases and indices in which the publication is indexed;
3. The authors authorize the Universidad Nacional de Colombia's journal Ingeniería e Investigación to publish the document in whatever required format (printed, digital, electronic or whatsoever known or yet to be discovered form) and authorize Ingeniería e Investigación to include the work in any indices and/or search engines deemed necessary for promoting its diffusion;
4. The authors accept that such authorization is given free of charge and they, therefore, waive any right to receive remuneration from the publication, distribution, public communication and any use whatsoever referred to in the terms of this authorization.
