Published

2017-01-01

Determination of leachate toxicity through acute toxicity using Daphnia pulex and anaerobic toxicity assays

Determinación de la toxicidad de lixiviados a través de toxicidad aguda utilizando Daphnia pulex y ensayos de toxicidad anaerobia

Keywords:

Daphnia pulex, leachate, municipal landfill, anaerobic toxicity (en)
Daphnia pulex, lixiviado, relleno sanitario municipal, toxicidad anaerobia (es)

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Authors

  • Patricia Torres Lozada Escuela de Ingeniería de Recursos Naturales y del Ambiente - EIDENAR Grupo de Investigación Estudio y Control de la Contaminación Ambiental - ECCA Facultad de Ingeniería Universidad del Valle https://orcid.org/0000-0001-9323-6677
  • Yazmín Stefhany Carabalí Rivera Escuela de Ingeniería de Recursos Naturales y del Ambiente - EIDENAR Grupo de Investigación Estudio y Control de la Contaminación Ambiental - ECCA Facultad de Ingeniería Universidad del Valle
  • Luz Edith Barba Ho Escuela de Ingeniería de Recursos Naturales y del Ambiente - EIDENAR Grupo de Investigación Estudio y Control de la Contaminación Ambiental - ECCA Facultad de Ingeniería Universidad del Valle
The municipal solid waste (MSW) of large cities, in particular in developing countries, is mainly disposed of in landfills (LFs), whose inadequate management generates the emission of greenhouse gases and the production of leachates with high concentrations of organic and inorganic matter and occasionally heavy metals. In this study, the toxicity of the leachates from an intermediate-age municipal landfill was evaluated by ecotoxicity and anaerobic digestion tests. The acute toxicity assays with Daphnia pulex presented a toxic unit (TU) value of 49.5%, which indicates that these leachates should not be directly discharged into water sources or percolate into the soil because they would affect the ecosystems served by these waters. According to statistical analyses, the leachate toxicity is mainly associated with the inorganic fraction, with chlorides, calcium hardness and calcium having the greatest influence on the toxicity. The anaerobic toxicity assays showed that in the exposure stage, the methanogenic activity exceeded that of the control, which suggests that the anaerobic bacteria easily adapted to the leachate. Therefore, this treatment could be an alternative to mitigate the toxicity of the studied leachates. The inhibition presented in the recovery stage, represented by a reduction of the methanogenic activity, could arise because the amount of supplied substrate was not enough to fulfill the carbon and nutrient requirements of the bacterial population present.
The municipal solid waste (MSW) of large cities, in particular the ones of developing countries, is mainly disposed in landfills (LFs), whose inadequate management generates the emission of greenhouse gases and the production of leachates with high concentrations of organic and inorganic matter and, occasionally heavy metals. In this study, the toxicity of the leachates from an intermediate-age municipal landfill was evaluated by ecotoxicity and anaerobic toxicity tests. The acute toxicity assays with Daphnia pulex presented a toxic unit (TU) value of 49.5%, which indicates that these leachates should not be directly discharged into water sources or percolate into the soil because they would affect the ecosystems related to these waters. According to statistical analyses, the leachate toxicity is mainly associated with the inorganic fraction, having the chlorides, calcium hardness and, calcium as the greatest influences on the toxicity. The anaerobic toxicity test showed that in the exposure stage, the methanogenic activity exceeded the control one, which suggests that the anaerobic bacteria easily adapted to the leachate. Therefore, this treatment could be an alternative to mitigate the toxicity of the studied leachates. The inhibition presented in the recovery stage, represented by a reduction of the methanogenic activity, may be because the amount of supplied substrate was not enough to fulfill the carbon and nutrient requirements of the bacterial population present.

References

Al-Wabel, M., Yehya, W., Farraj, A., Maghraby, S. (2011). Charac-teristics of landfill leachates and bio-solids of municipal solid waste (MSW) in Riyadh City, Saudi Arabia. Journal of the Saudi Society of Agricultural Sciences, 10 (2), 65–70.

Anthonisen, A., Loehr, R., Prakasam, T. (1976). Inhibition of nitrifica-tion by ammonia and nitrous-acid. Journal water pollution con-trol federation, 48 (5), 835-852.

APHA, AWWA, WEF, American Public Health Association. (2012).Standard Methods for the Examination of Water and Wastewater. 22nd ed. Washington DC., USA.

Asselman, J., Janssen, C., Smagghe, G., DeE Schamphelaeres, K. (2014). Ecotoxicity of binary mixtures of Microcystis aeruginosa and insecticides to Daphnia pulex. Environmental Pollution, 188, 56-63.

Atwater, J; Jasper, S; Mavinic, D; Koch, F. (1983). Experiments using Daphnia to measure landfill leachate toxicity. Water Re-search, 17 (12), 1855-1861.

Aziz, S., Aziz, H., Yusoff, M., Bashir, M., Umar, M. (2010). Leachate characterization in semi-aerobic and anaerobic sanitary land-fills: A comparative study. Journal Environmental Management, 91 (12), 2608-2614.

Barata, C., Baird, D., Nogueira, A., Soares, A., Riva, M. (2006). Toxicity of binary mixtures of metals and pyrethroid insecticides of Daphnia magna Straus. Implications for multi-substance risks assessment. Aquatic Toxicology, 78 (1), 1-14.

Boumechhour, F., Rabah, K., Lamine, C., Said, B. (2013). Treatment of landfill leachate using Fenton process and coagula-tion/flocculation. Water Environmental Journal, 27 (1), 114-119.

Chen, Y., Cheng, J., Creamer, K. (2008). Inhibition of anaerobic digestion process: A review. Bioresource Technology, 99 (10), 4044-4064.

Cho, E., Tameda, K., Hanashima, M., Yamada, T., HIGUCHI, S. (2009). Toxicological evaluation of the chemical oxidation methods for landfill stabilization. Waste Management, 29 (3), 1006-1011.

El-Fadel, M., Bou-Zeid, E., Chahine, W., Alayli, B. (2002). Temporal variation of leachate quality from pre-sorted and baled munic-ipal solid waste with high organic and moisture content. Waste Management, 22 (3), 269-282.

Eriksson, O., Bisaillon, M. (2011). Multiple system modelling of waste management. Waste Management, 31 (12), 2620-2630.

Fátima, D., Tavares, L., Magalhães, F., Pereira, C., Souza, C., Tava-reS, A. (2012). Caracterização e percepção ambiental dos re-síduos sólidos urbanos nas diferentes classes sociais no municí-pio de alfenas – MG. Agronegócios e Meio Ambiente, 5, 25-49.

Fernández, G., Vázquez, E., Martínez, P. (2002). Inhibidores del proceso anaerobio: compuestos utilizados en porcicultura. In-geniería, 6 (3), 67-71.

Field, J., Medición de parámetros, manual de arranque y opera-ción de Sistemas de Flujo Ascendente con manto de lodo– UASB, Universidad del Valle, CVC, Universidad Agrícola de Wageningen, Colombia, 1995.

Gallegos, M., Celis, L., Razo, E. (2010). Competencia por sustrato durante el desarrollo de biomasa sulfatorreductora a partir de un lodo metanogénico en un reactor UASB. Revista Internac-ional de Contaminación Ambiental, 26 (2), 109–117.

Hoornweg, D., Bhada-Tata, P., What a waste: A global review of solid waste management, Urban Development and Local Gov-ernment Unit - World Bank, Washington D.C., 2012.

Isidori, M., Lavorgna, M., Nardelli, A., Parrella, A. (2003). Toxicity identification evaluation of leachates from municipal solid waste landfills: a multispecies approach. Chemosphere, 52 (1), 85–94.

Jemec, A., tišler, T., Žgajnar-Gotvajn, A. (2012). Assessment of landfill leachate toxicity reduction after biological treatment. Archives of Environmental Contamination and Toxicology, 62 (2), 210-221.

JING, Z., Hu, Y., Niu, Q., Liu, Y., Li, Y., Wang, X. (2013). UASB perfor-mance and electron competition between methane-producing archaea and sulfate-reducing bacteria in treating sulfate-rich wastewater containing ethanol and acetate. Biore-source Technology, 137, 349–357.

Kheradmand, S., Karimi-Jashni, A., Sartaj, M. (2010). Treatment of municipal landfill leachate using a combined anaerobic di-gester and activated sludge system. Waste Management, 30 (6), 1025–1031.

Kulikowska, D., Klimiuk, E. (2008). The effect of landfill age on municipal leachate composition. Bioresource Technology, 99 (13), 5981–5985.

Lee, A.H., Nikraz, H., Hung, Y.T. (2010). Influence of Waste Age on Landfill Leachate Quality. International Journal of Environmental Science and Development, 1 (4), 347-350.

Kjeldsen, P., Barlaz, M., Rooker, A., Baun, A., Ledin, A., Christensen, T. (2002). Present and long-term composition of MSW landfill leachate: a review. Critical Reviews in Environmental Science and Technology, 32 (4), 297-336.

Moeinaddini, M., Khorasani, N., Danehkar, A., Darvishsefat, A., Zienalyan, M. (2010). Siting MSW landfill using weighted linear combination and analytical hierarchy process (AHP) method-ology in GIS environment (case study: Karaj). Waste Manage-ment, 30 (5), 912-920.

Olivero, J., Padilla, C., De la Rosa, O. (2008). Relationships be-tween physicochemical parameters and the toxicity of leacha-tes from a municipal solid waste landfill. Ecotoxicology and Envi-ronmental Safety, 70 (2), 294–299.

Öman, C., Junestedt, C. (2008). Chemical characterization of landfill leachates- 400 parameters and compounds. Waste Ma-nagement, 28 (10), 1876-1891.

Pablos, M., Maritini, F., Fernández, C., BABIN, M., Herraez, I., Miran-da, J., Martínez, J., Carbonell, G., Garcia, P., Tarazona, J. (2011). Correlation between physicochemical and ecotoxicological approaches to estimate landfill leachates toxicity. Waste Man-agement, 31 (18), 1841–1847.

Pivato, A., Gaspari, L. (2006). Acute toxicity test of leachates from traditional and sustainable Landfills using luminescent bacteria. Waste Management, 26 (10), 1148–1155.

Ramírez-Sosa, D., Castillo-Borges, E., Méndez-Novelo, R., Sauri-Riancho, M., Barceló-Quintal, M., Marrufo-Gómez, J. (2013). De-termination of organic compounds in landfill leachates treated by Fenton–Adsorption. Waste Management, 33 (2), 390-395.

Renou, S., Givaudan, J.G., Poulain, S., DIRASSOUYAN, F., MOULIN, P. (2008). Landfill leachate treatment: Review and opportunity. Journal of Hazardous Materials, 150 (3), 468–493.

Ribé, V., Nehrenheim, E., Odlare, M., Gustavsson, L., Berglind, R., Forsberg, Å. (2012). Ecotoxicological assessment and evaluation of a pine bark biosorbent treatment of five landfill leachates. Waste Management, 32 (10), 1886-1894.

Rivera-Laguna, E., Barba, L., Torres, P. (2013). Determinación de la toxicidad de lixiviados provenientes de residuos sólidos urbanos mediante indicadores biológicos. Afinidad, LXX (563), 183-188.

Romero, C. (2010). Aprovechamiento integral de lixiviados (PhD thesis, Ingeniería Química, Departamento de Ingeniería Química y Textil). Universidad de Salamanca.

Saucedo, G., Piña, O., Rodríguez, R., Cruz, Y. Degradación y estabilización acelerada de residuos sólidos urbanos (RSU) por tratamientos aerobios y anaerobios. Universidad Autónoma Metropolitana, Unidad Iztapalapa, Casa abierta al campo, Mé-xico, 2007.

Shouliang, H., Beidou, X., Haichan, Y., Liansheng, H., Shilei, F., Hongliang, L. (2008). Characteristics of dissolved organic matter (DOM) in leachate with different landfill ages. Journal of Envi-ronmental Sciences, 20 (4), 492-498.

Silva, J., Torrejón, G., Bay-Schmith, E., Larrain, A. (2003). Calibra-ción del Bioensayo de Toxicidad aguda con Daphnia pulex (Crustáceo: Cladócera) usando un toxico de referencia. Ga-yana, 67 (1), 87-96.

SSPD. Estudio Sectorial de Aseo 2006-2009. Superintendencia de Servicios Públicos Domiciliarios, Imprenta Nacional, Bogotá, Colombia. 2010.

Tisler, T., Zagorc-Koncan, J. (1997). Comparative Assessment or Toxicity of Phenol, Formaldehyde, and Industrial Wastewater to Aquatic. Water, Air, and Soil Pollution, 97, 315-322.

Torres-Lozada, P., Barba-Ho, L.E, Rodríguez-victoria, J.A, Marmole-jo-Rebellon, L.F., Pizarro-Loaiza, C.A (2010). Influencia de la in-corporación de lixiviados sobre la biodegradabilidad anaero-bia de aguas residuales domésticas. Revista Ingeniería e Inves-tigación, 30 (1), 75 – 79.

Torres, P., Barba, L., Ojeda, C., Martínez, J., Castaño, Y. (2014). Influencia de la edad de lixiviados sobre su composición físico-química y su potencial de toxicidad. Revista U.D.C.A. Actuali-dad y Divulgación Científica, 17 (1), 245-255.

USEPA – UNITED STATES ENVIRONMENTAL PROTECTION AGENCY, Methods for measuring the acute toxicity of effluents and re-ceiving waters to freshwater and marine organism. Fourth Edi-tion. Report 600/4-90/027F, USA, 1990.

Wiszniowski, J., Robert, D., Surmacz-Gorska, J., Miksch, K., Weber, J. (2006). Landfill leachate treatment methods: A review. Envi-ronmental Chemistry Letters, 4 (1), 51-61.

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