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Detección y cuantificación de hidrocarburos en agua y sedimentos por medio de fluorescencia usando un espectrofluorómetro comercial
Approach to the Detection and Quantification of Hydrocarbons in Water and Sediments by Fluorescence Using a Commercial Spectrofluorometer
Detecção e quantificação de hidrocarbonetos em água e sedimentos por fluorescência usando um espectrofluorômetro comercial
DOI:
https://doi.org/10.15446/rev.colomb.quim.v53n2.115720Palabras clave:
Hidrocarburos aromáticos policíclicos (HAP), Contaminación ambiental, Detección de bajo costo, Fluorescencia (es)Polycyclic Aromatic Hydrocarbons (HAP), Environmental Pollution, Low-cost Detection, Fluorescence (en)
Hidrocarbonetos policíclicos aromáticos-HAPs, Poluição ambiental, Detecção de baixo custo, Fluorescência (pt)
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En este estudio se utilizó un espectrofluorómetro comercial (Cytation 3) para detectar hidrocarburos en muestras contaminadas artificialmente (dopadas) con agua y sedimentos por medio de fluorescencia de onda fija. Se identificaron los espectros de emisión y las señales de fluorescencia en pico de antraceno, fenantreno, pireno y fluoranteno con límites de detección 19, 89, 26 y 35 ppb, respectivamente. La recuperación de hidrocarburos en muestras dopadas de agua estuvo entre 86 y 100% individualmente, aunque en mezcla se observaron interferencias de señales por la cercanía en los picos de emisión generando aditividad. En muestras de sedimentos, la recuperación de hidrocarburos estuvo entre 10 y 25%, y hasta un 36% en mezcla.
Los resultados indican que la detección individual de los hidrocarburos es factible con base en las señales detectadas y las cuantificaciones realizadas; sin embargo, es necesario investigar interacciones con otros compuestos y su comportamiento en muestras dopadas o muestras de sitios con alta contaminación obtenidas en campo para validar su aplicación en el monitoreo ambiental.
In this study, a commercial spectrofluorometer (Cytation 3) was employed to detect hydrocarbons in artificially contaminated (doped) water and sediment samples using fixed wavelength fluorescence. Emission spectra and peak fluorescence signal of anthracene, phenanthrene, pyrene, and fluoranthene were identified with detection limits of 19, 89, 26, and 35 ppb, respectively. The recovery of hydrocarbons in contaminated water samples ranged between 86 and 100% individually, although signal interferences were observed in mixture due to the proximity of the emission peaks, which generated additivity. In sediment samples, hydrocarbon recovery ranged from 10 to 25% and up to 36% in the mixture.
The results indicate that the individual detection of hydrocarbons is feasible based on the signals detected and the quantifications performed, however, it is necessary to investigate interactions with other compounds and their behavior in doped samples or samples from highly contaminated sites obtained in the field to validate their application in environmental monitoring.
Neste estudo, um espectrofluorômetro comercial (Cytation 3) foi empregado para detectar hidrocarbonetos em amostras de água e sedimentos artificialmente contaminados por fluorescência de comprimento de onda fixo. Foram identificados os espectros de emissão e sinais de pico de fluorescência de antraceno, fenantreno, pireno e fluoranteno, com limites de detecção de 19, 89, 26 e 35 ppb, respectivamente. A recuperação de hidrocarbonetos em amostras de água contaminada variou entre 86 e 100% individualmente, mas foram observadas interferências de sinal devido à proximidade dos picos de emissão, o que gerou aditividade. Em amostras de sedimentos, as recuperações foram entre 10 e 25% e até 36% na mistura.
Os resultados indicam que a detecção individual de hidrocarbonetos é viável com base nos espectros detectados e nas quantificações realizadas. No entanto, é necessário investigar as interações com outros compostos e seu comportamento em amostras reais obtidas em locais altamente contaminados, a fim de validar sua aplicação no monitoramento ambiental.
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Derechos de autor 2025 Diego Mora Solarte, Angélica González Reina, Ivonne Calderón Delgado, Yohana Velasco Santamaría
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